TAS2R expression profile in brown adipose, white adipose, skeletal muscle, small intestine, liver and common cell lines derived from mice
TAS2R expression profile in brown adipose, white adipose, skeletal muscle, small intestine, liver and common cell lines derived from mice
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DOI：
10.1016/j.genrep.2020.100763
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发表时间：
2020-09-01
期刊：
影响因子：
1.3
通讯作者：
Kato, Eisuke
中科院分区：
文献类型：
--
作者：
Kimura, Shunsuke;Kato, Eisuke
味觉2受体(TAS2Rs)由G蛋白偶联受体家族组成，其中25个亚型和35个亚型分别在人类和小鼠中被鉴定。TAS2Rs是一种口头表达的受体，负责感知苦味化合物和苦味。在过去的十年中，研究发现TAS2R在一些口外组织中的表达以及TAS2R在呼吸道和肠道中的作用，而它们在其他口外器官中的表达和功能在很大程度上是未知的。在这项研究中，我们分析了来自棕色脂肪、白色脂肪、骨骼肌、小肠和肝脏的小鼠总RNA以及两个常见的小鼠来源细胞系(3T3-L1和C2C12)中TAS2Rs的mRNA表达。TAS2R中棕色脂肪9例，白色脂肪11例，骨骼肌8例，小肠10例，肝组织8例。两种细胞系均表达多个反映相应组织表达的TAS2Rs。这些结果表明，TAS2Rs可能在相应的器官中具有功能，3T3-L1和C2C12可能是探索TAS2Rs功能的模型细胞系。
The taste 2 receptors (TAS2Rs) comprise a family of G-protein-coupled receptors, of which 25 subtypes and 35 subtypes have been identified in humans and mice, respectively. TAS2Rs are orally expressed receptors responsible for perceiving bitter compounds and sensing bitter tastes. Over the last decade, investigations have uncovered TAS2R expression in some extraoral tissues and roles for TAS2Rs in the airways and gut, while their expression and function in other extraoral organs have been largely unexplored. In this study, we analyzed the mRNA expression of TAS2Rs in mice total RNA derived from brown adipose, white adipose, skeletal muscle, small intestine and liver, along with two common mouse-derived cell lines (3T3-L1 and C2C12). Nine members of TAS2R were characterized in brown adipose, 11 in white adipose, 8 in skeletal muscle, 10 in small intestine, and 8 in liver tissues. Both cell lines were found to express multiple TAS2Rs reflecting the expression of corresponding tissues. These results suggest that TAS2Rs may have functions in the corresponding organs and that 3T3-L1 and C2C12 may be useful as model cell lines to explore the functions of TAS2Rs.