The effect of size on uptake of orally administered latex microparticles in the small intestine and transport to mesenteric lymph nodes
The effect of size on uptake of orally administered latex microparticles in the small intestine and transport to mesenteric lymph nodes
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DOI：
10.1023/a:1016064320334
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发表时间：
1996-08-01
影响因子：
3.7
通讯作者：
Hodges, GM
中科院分区：
文献类型：
--
作者：
Carr, KE;Hazzard, RA;Hodges, GM
目的：本研究探讨了胃肠道粘膜屏障的大小和颗粒通过胃肠道其他部位的转运之间的关系。方法建立幼年大鼠荧光乳胶颗粒模型，用荧光显微镜计数给药后0.5h小肠和肠系膜淋巴组织中的颗粒数量。结果大体组织分析证实Peyer‘s斑片区存在各种大小的颗粒，而结节组织中只有2um m的颗粒。显微镜检查证实大多数肠道和结节组织部位和隔室均可摄取2微米和6微米的颗粒。相反，较大颗粒的摄取大大减少。结论虽然较小(2微米)的颗粒被更多地摄取，尤其是被上皮组织摄取，但就转移到淋巴结的体积而言，6微米大小似乎更有效。这可能会对用于药物和疫苗输送的颗粒的治疗使用以及辐射安全产生影响。
Purpose, The present study examines the relationship between size and particle transit across the mucosal barrier of the gastrointestinal tract to other sites of the body. The extent of particle uptake with increasing size, the tissue distribution and cut-off points for 2-20 mu m particles is investigated.Methods, An established fluorescent latex particle-young adult rat model is used and particle numbers in small intestine and mesenteric lymph nodes, 0.5 h post administration, counted by fluorescence microscopy in bulk tissue specimens and cryosections.Results, Bulk tissue analysis provides evidence for the presence of particles of all sizes in the Peyer's patch regions, but only for 2 mu m particles in the nodal tissues. Microscopy establishes uptake of both 2 and 6 mu m particles in most intestinal and nodal tissue sites and compartments. By contrast, uptake of the larger particles is much reduced.Conclusions, Although more of the smaller (2 mu m) particles are taken up, particularly by epithelial tissues, the 6 mu m size appears more efficient in terms of volume translocated to lymph nodes. This could have implications in the therapeutic use of particles for drug and vaccine delivery and for radiation safety.