Identification of a major QTL and associated molecular marker for high arabinoxylan fibre in white wheat flour
Identification of a major QTL and associated molecular marker for high arabinoxylan fibre in white wheat flour
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DOI：
10.1371/journal.pone.0227826
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发表时间：
2020-02-05
期刊：
影响因子：
3.7
通讯作者：
Griffiths, Simon
中科院分区：
文献类型：
--
作者：
Lovegrove, Alison;Wingen, Luzie U.;Griffiths, Simon
膳食纤维（DF）具有多种健康益处，小麦谷物是人体健康DF的主要来源。然而，DF在白色小麦粉中被耗尽，而白小麦粉比全谷物更广泛地消费。白色面粉中的主要DF组分是细胞壁多糖阿拉伯木聚糖（AX）。我们已经鉴定出中国小麦品种豫麦34具有异常高的水溶性和不溶性形式的AX含量。因此，我们使用了豫麦34和其他四个小麦品种之间的杂交产生的群体，三个具有平均AX含量（Ukrainka，Alfred和Claire）和一个也具有异常高的AX（Valoris），为了定位可溶性AX（测定为全麦面粉的水提取物的相对粘度）和总AX（通过白色面粉的酶指纹图谱测定）的QTL。一些QTL被定位，但大多数只检测到一个或两个组合。然而，所有四个组合都显示了1B染色体上高RV/总AX的强QTL，豫麦34是递增亲本，并且通过分析来自豫麦34但通过生化分析选择的高AX系验证了豫麦34高AX等位基因的KASP标记。在豫麦34 × Valoris的6 B染色体上也定位了一个RV的QTL，Valoris是递增等位基因，这与该群体的超亲分离现象相一致。在一个独立的种质小组的关联研究确定了标记性状协会在这些相同的位置相对粘度，而直接选择纤维含量育种导致高水平的富集豫麦34 1B等位基因。因此，数据表明，标记辅助育种可用于开发在白色面粉中具有高AX纤维的小麦。
Dietary fibre (DF) has multiple health benefits and wheat grains are major sources of DF for human health. However, DF is depleted in white wheat flour which is more widely consumed than wholegrain. The major DF component in white flour is the cell wall polysaccharide arabinoxylan (AX). We have identified the Chinese wheat cultivar Yumai 34 as having unusually high contents of AX in both water-soluble and insoluble forms. We have therefore used populations generated from crosses between Yumai 34 and four other wheat cultivars, three with average contents of AX (Ukrainka, Altigo and Claire) and one also having unusually high AX (Valoris), in order to map QTLs for soluble AX (determined as relative viscosity of aqueous extracts of wholemeal flours) and total AX (determined by enzyme fingerprinting of white flour). A number of QTL were mapped, but most were only detected in one or two crosses. However, all four crosses showed strong QTLs for high RV/total AX on chromosome 1B, with Yumai 34 being the increasing parent, and a KASP marker for the Yumai 34 high AX allele was validated by analysis of high AX lines derived from Yumai 34 but selected by biochemical analysis. A QTL for RV was also mapped on chromosome 6B in Yumai 34 x Valoris, with Valoris being the increasing allele, which is consistent with the observation of transgressive segregation for this population. Association studies in an independent germplasm panel identified marker trait associations for relative viscosity in these same locations while direct selection for fibre content in breeding resulted in high levels of enrichment for the Yumai 34 1B allele. The data therefore indicate that marker-assisted breeding can be used to develop wheat with high AX fibre in white flour.