Overproduction of Mitochondrial Fission Proteins in Membranous Nephropathy in Children.
Overproduction of Mitochondrial Fission Proteins in Membranous Nephropathy in Children.
复制标题
儿童膜性肾病中线粒体裂变蛋白的过度产生。
DOI：
10.1159/000496006
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Zhu Sai-Nan
中科院分区：
文献类型：
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作者：
Wei Qi Jiao;Xu Han;Guan Na;Ren Ya-Li;Liu Xiao-Ya;Wu Guo-Hong;Zhu Sai-Nan
背景/目的肾病综合征（NS）的分子机制尚未完全阐明。线粒体分裂蛋白被发现参与体外足细胞损伤。在我们以前的研究中发现阿霉素肾病肾小球线粒体分裂蛋白表达增加。线粒体分裂蛋白是否参与NS足细胞损伤尚不清楚。本研究探讨了动力蛋白相关蛋白1（dynamin-related protein 1，Drp 1）和线粒体分裂蛋白1（mitochondrial fission protein 1，Fis 1）在NS患儿肾小球中的表达及其可能的病理意义。包括6名孤立性血尿儿童和3名正常对照。肾小球表达的Drp 1，磷酸化Drp 1（Ser 616）和Fis 1，尿蛋白测量，足细胞线粒体密度在电子显微镜下进行了调查和compared.ResultsGlomerular表达的Drp 1，磷酸化Drp 1（Ser 616）和Fis 1主要是增加在儿童NS膜性肾病。肾小球表达的Drp 1，磷酸化Drp 1（Ser 616）和Fis 1和足细胞线粒体密度或尿蛋白measurement.Conclusionglomerular过量生产的Drp 1，磷酸化Drp 1（Ser 616）和Fis 1主要发生在儿童膜性肾病。其病理意义值得进一步探讨。
Background/AimsThe molecules involved in nephrotic syndrome (NS) have not been fully clarified. Mitochondrial fission proteins are found to be involved in podocyte injury in vitro. Increased glomerular expression of mitochondrial fission proteins was found in adriamycin nephropathy in our previous study. Whether or not mitochondrial fission proteins are involved in podocyte injury in NS is not clear. This study explored the glomerular expression and possible pathological significance of mitochondrial fission-associated proteins, including dynamin-related protein 1 (Drp1) and mitochondrial fission protein 1 (Fis1), in children with NS.MethodsEighteen children with primary NS, including 6 with minimal change disease, 6 with focal segmental glomerulosclerosis, 6 with membranous nephropathy, 6 children with isolated haematuria and 3 normal controls were included. The glomerular expression of Drp1, phospho-Drp1 (Ser616) and Fis1, urinary protein measurements, and podocyte mitochondrial density under electron microscopy were investigated and compared.ResultsGlomerular expression of Drp1, phospho-Drp1 (Ser616) and Fis1 was mainly increased in children with NS with membranous nephropathy. No relationship was found between glomerular expression of Drp1, phospho-Drp1 (Ser616) and Fis1 and podocyte mitochondrial density or urinary protein measurements.ConclusionGlomerular overproduction of Drp1, phospho-Drp1 (Ser 616) and Fis1 occurred mainly in children with membranous nephropathy. The pathological significance deserves further investigation.