The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes.
The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes.
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人类皮肤中表达的 II 型角蛋白基因的序列：所有中间丝基因的结构保守性。
DOI：
10.1073/pnas.82.14.4683
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发表时间：
1985
影响因子：
11.1
通讯作者：
Fuchs,E
中科院分区：
文献类型：
--
作者：
Tyner,AL;Eichman,MJ;Fuchs,E
我们在这里报告的56 kDa的II型角蛋白（指定K6 b）的基因的编码序列。使用一个亚克隆特异性的一个独特的3'非编码区的编码的mRNA，我们已经表明，该基因是至少两个56 kDa角蛋白基因在人类表皮中表达丰富。分割该基因的编码部分是8个内含子，其中6个与远亲III型中间丝基因（波形蛋白）的内含子位置相同，其中5个与远亲I型基因（50 kDa角蛋白）的内含子位置相同。这些结果表明，所有三类中间丝基因的共同祖先起源。所有高度保守的内含子位置都位于所有中间丝多肽共有的四个中央α-螺旋结构域内，但不划分，这表明这些基因可能不是通过重组介导的分离结构域的连接而零碎地产生的，因为它们目前是已知的。
We report here the coding sequence of the gene for a 56-kDa type II keratin (designated K6b). Using a subclone specific for a unique 3' noncoding region of the encoded mRNA, we have shown that this gene is one of at least two 56-kDa keratin genes expressed in abundance in human epidermis. Segmenting the coding portion of this gene are eight introns, six of which are identically positioned with those of a distantly related type III intermediate filament gene (vimentin), and five of which are identically positioned with those of a distantly related type I gene (50-kDa keratin). These results indicate a common ancestral origin for all three classes of intermediate filament genes. All of the highly conserved intron positions are located within, but do not demarcate, the four central alpha-helical domains common to all intermediate filament polypeptides, suggesting that these genes were probably not created piecemeal by recombination-mediated linkage of separate structural domains as they presently are known.