A strategy for programming the regulation of in vitro transcription with application in molecular circuits
A strategy for programming the regulation of in vitro transcription with application in molecular circuits
复制标题
体外转录调控编程策略及其在分子电路中的应用
DOI：
10.1039/d0nr08465d
复制
发表时间：
2021
期刊：
影响因子：
6.7
通讯作者：
Pan Linqiang
中科院分区：
文献类型：
--
作者：
Hu Yingxin;Xie Chun;Xu Fei;Pan Linqiang
In vitro transcription is a convenient platform for fabricating nanodevices and has been used for assembling synthetic networks. However, it remains challenging to regulate synthetic cell-free in vitro transcription by multiple stimuli in a simple and programmable way. We proposed a strategy to regulate in vitro transcription by controlling the transcription templates’ promoter domain via variable DNA inputs. To demonstrate the utility of this strategy, various logic circuits and cascading circuits were implemented. With the advantage of simplicity, modularity, programmability, and extensibility, the proposed strategy has potential in biocomputing, bioanalytical, and therapeutic applications.
