Mutations in ras genes in cells cultured from mouse skin tumors induced by ultraviolet irradiation.
Mutations in ras genes in cells cultured from mouse skin tumors induced by ultraviolet irradiation.
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紫外线照射诱导小鼠皮肤肿瘤培养细胞中 ras 基因突变。
DOI：
10.1073/pnas.91.15.7189
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发表时间：
1994
影响因子：
11.1
通讯作者：
H. Takebe
中科院分区：
文献类型：
--
作者：
C. Nishigori;S. Wang;J. Miyakoshi;M. Sato;T. Tsukada;T. Yagi;S. Imamura;H. Takebe
在近紫外线照射诱导的小鼠皮肤肿瘤培养细胞中检测到ras癌基因突变。将从紫外线诱导的肿瘤细胞中提取的DNA转染到金黄地鼠胚胎细胞中，在26个细胞株中的22个中证实了病灶形成能力，其中15个具有重复的小鼠序列。在这22个细胞株中，有10个细胞株检测到小鼠ras基因。ras基因的点突变位于Ha-ras密码子13（2株GGC->GTC，1株GGC->AGC）、Ki-ras密码子61（2株CAA->GAA）和N-ras密码子61（2株CAA->CAT，2株CAA->AAA）。在一种肿瘤细胞株中，没有指示碱基变化。大多数突变发生在二嘧啶位点。嘧啶二聚体或嘧啶（6-4）嘧啶酮光产物是皮肤癌的可能原因。碱基的变化主要发生在G. C碱基对上，颠换占优势。
Mutations in ras oncogenes were detected in cultured cells of mouse skin tumors induced by near-UV irradiation. DNA extracted from the UV-induced tumor cells was transfected to golden hamster embryo cells, and focus-forming ability was confirmed in 22 of 26 cell strains, 15 of which had the repetitive mouse sequence. Mouse ras genes were detected in 10 of these 22 cell strains. Point mutations in the ras genes were at Ha-ras codon 13 (GGC-->GTC in two strains, GGC-->AGC in one strain), Ki-ras codon 61 (CAA-->GAA in two strains), and N-ras codon 61 (CAA-->CAT in two strains, CAA-->AAA in two strains). In one tumor cell strain no base change was directed. Most mutations occurred at dipyrimidine sites. Pyrimidine dimers or pyrimidine(6-4)pyrimidone photoproducts are the likely cause of the skin cancers. The base change occurred preferentially at G.C base pairs, and transversions predominated.