miR-542-3p suppresses colorectal cancer progression through targeting survivin
miR-542-3p suppresses colorectal cancer progression through targeting survivin
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DOI：
10.21037/11243
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发表时间：
2016-12
影响因子：
0.9
通讯作者：
Chunxiang Ye;Guanjun Yue;Zhanlong Shen;Bo Wang;Yang Yang-Yang;Tao Li;Shuqiang Mao;K. Jiang;Y. Ye-Y
中科院分区：
文献类型：
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作者：
Chunxiang Ye;Guanjun Yue;Zhanlong Shen;Bo Wang;Yang Yang-Yang;Tao Li;Shuqiang Mao;K. Jiang;Y. Ye-Y
背景：已有报道称，miR-542-3p在多种肿瘤类型中具有肿瘤抑制作用，但其在结直肠癌中的作用尚不完全清楚。本研究旨在探讨miR-542-3p在人结直肠癌中的表达及其可能的作用。
方法：采用实时荧光定量聚合酶链式反应(Real-Time-PCR)检测结直肠癌患者组织和血浆中miR-542-3p的表达。通过体外功能测定评价miR-542-3p对结直肠癌细胞侵袭表型的影响。荧光素酶活性测定证实miR-542-3p与Survivin直接结合。
结果：转移灶来源的大肠癌细胞株中MIR-542-3p表达下调。65例结直肠癌患者中，63.08%(41/65)的癌组织miR-542-3p表达降低。MiR-542-3p的表达与淋巴血管侵犯(P=0.008)、远处转移(P=0.006)、肿瘤分期(P=0.034)和患者的生存期(P=0.027)有关。IV期患者血浆miR-542-3p表达降低。体内外实验表明，miR-542-3P能抑制大肠癌细胞的侵袭性表型。最后确定Survivin是miR-542-3p的直接靶点。
在CRC。
结论：miR-542-3p在结直肠癌中的低表达与患者不良的临床病理特征有关。MIR-542-3P抑制大肠癌细胞株的侵袭性表型。Survivin是miR-542-3p在结直肠癌中的直接靶点。
Background: miR-542-3p has been reported to be a tumor suppressor in several tumor types, while its role in colorectal cancer (CRC) has not been fully understood. This study aimed to investigate the expression of miR-542-3p and its potential role in human CRC.
Methods: Real-time PCR was used to detect the expression of miR-542-3p in tissues and plasma of CRC patients. The impact of miR-542-3p on the aggressive phenotypes of CRC cells were evaluated by in vitro functional assays. Luciferase activity assay was conducted to confirm the direct binding of miR-542-3p to survivin.
Results: miR-542-3p was decreased in CRC cell lines that derived from metastatic sites. Among the 65 CRC patients enrolled in this study, 63.08% (41/65) had a decreased miR-542-3p expression in cancerous tissues. miR-542-3p expression was associated with lymphovascular invasion (P=0.008), distant metastasis (P=0.006), tumor stage (P=0.034) and patients’ survival (P=0.027). A decreased expression of miR-542-3p in plasma was detected in stage IV patients. In vitro and in vivo experiments showed that miR-542-3p could inhibit the aggressive phenotypes of CRC cells. Finally, survivin was identified as a direct target of miR-542-3p
in CRC.
Conclusions: Decreased expression of miR-542-3p in CRC patients was associated with unfavourable clinicopathological features of the patients. miR-542-3p inhibits the aggressive phenotypes of CRC cell lines. Survivin is a direct target of miR-542-3p in CRC.