Effects of diameters and crystals of titanium dioxide nanotube arrays on blood compatibility and endothelial cell behaviors
Effects of diameters and crystals of titanium dioxide nanotube arrays on blood compatibility and endothelial cell behaviors
复制标题
二氧化钛纳米管阵列直径和晶体对血液相容性和内皮细胞行为的影响
DOI：
10.1016/j.colsurfb.2019.110521
复制
发表时间：
2019-12-01
影响因子：
5.8
通讯作者：
Pan, Changjiang
中科院分区：
文献类型：
--
作者：
Gong, Zhihao;Hu, Youdong;Pan, Changjiang
二氧化钛纳米管阵列（TNTA）因其独特的管状结构和良好的生物相容性而在生物材料和生物医学领域引起了广泛的关注。本文通过阳极氧化在钛表面原位制备了不同纳米管直径和长度的TNTA，并通过退火处理进一步改变了其晶体结构。研究了不同直径和晶体的TNTA对血液相容性和内皮细胞行为的影响。结果表明，通过控制阳极氧化电压可以得到直径为30～90 nm的TNTA，且退火处理没有明显改变纳米管阵列的直径和长度。然而，退火处理可以将非晶态TNTA转变为锐钛矿结构。纳米管阵列的直径和晶体结构对表面润湿性和蛋白质吸附起着关键作用。与原始钛相比，较大直径的纳米管阵列表现出更好的表面亲水性，并且退火处理进一步增强了亲水性。与原始钛相比，纳米管阵列结构具有选择性蛋白质吸附的特性，纳米管阵列可以促进牛血清白蛋白（BSA）的吸附并阻止纤维蛋白原（FIB）的吸附，但纳米管直径的增加会减少BSA的吸附并增加FIB的吸附。此外，锐钛矿结构的纳米管阵列可以促进BSA吸附，同时减少FIB吸附。因此，较小直径的TNTA和锐钛矿晶体具有良好的血液相容性和细胞相容性，它们不仅可以降低血小板粘附和溶血率，而且可以增加内皮细胞的粘附和增殖。总之，本研究的纳米管阵列可用于改善钛植入物的细胞相容性和血液相容性。
Titanium dioxide nanotube arrays (TNTAs) have attracted extensive attention in the fields of biomaterials and biomedicine due to their unique tubular structure and good biocompatibility. In this paper, TNTAs with different nanotube diameters and lengths were in situ prepared on the titanium surface by the anodic oxidation, and their crystal structures were further changed by annealing treatment. The effects of TNTAs with different diameters and crystals on the blood compatibility and endothelial cell behaviors were investigated. The results showed that TNTAs with the diameter of 30 similar to 90 nm can be obtained by controlling the anodization voltage, and annealing treatment did not obviously change the diameters and lengths of the nanotube arrays. However, annealing treatment can transform the amorphous TNTAs into the anatase structure. The diameter and crystal structure of the nanotube arrays played a key role in the surface wettability and protein adsorption. The nanotube array with larger diameter displayed better surface hydrophilicity as compared to the pristine titanium, and annealing treatment further enhanced the hydrophilicity. As compared to the pristine titanium, the nanotube array structure had the characteristic of selective protein adsorption, and the nanotube array can promote the bovine serum albumin (BSA) adsorption and prevent the fibrinogen (FIB) adsorption, however, the increase of nanotube diameter could reduce BSA adsorption and increase FIB adsorption. Besides, the nanotube array with anatase structure can promote BSA adsorption while reduce FIB adsorption. Therefore, the TNTAs with smaller diameter and anatase crystal had good blood compatibility and cell compatibility, they can not only reduce platelet adhesion and hemolysis rate but also increase endothelial cell adhesion and proliferation. In conclusion, the nanotube arrays of the present study can be used to improve the cell compatibility and blood compatibility of the titanium implants.