Chronic kidney disease alters intestinal microbial flora
Chronic kidney disease alters intestinal microbial flora
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DOI：
10.1038/ki.2012.345
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发表时间：
2013-02-01
影响因子：
19.6
通讯作者：
Andersen, Gary L.
中科院分区：
文献类型：
--
作者：
Vaziri, Nosratola D.;Wong, Jakk;Andersen, Gary L.
肠道中的微生物（微生物组）。是一个具有营养和保护功能的共生生态系统。由于生物化学环境塑造了微生物组的结构和功能，我们测试了慢性肾脏疾病中的尿毒症和/或饮食和药物干预是否会改变微生物组。为了鉴定不同的微生物种群，从24例终末期肾病（ESRD）患者和12名健康人的粪便中分离微生物DNA，并通过系统发育微阵列进行分析。ESRD组和对照组之间190个细菌操作分类单位（OTU）的丰度存在显著差异。ESRD患者中来自短杆菌属、链杆菌属、肠杆菌科、盐单胞菌科、莫拉菌科、Nesterenkonia、多囊菌科、假单胞菌科和硫杆菌科的OTU显著增加。为了将尿毒症的影响与个体间差异、共病状况以及饮食和药物干预分离，在5/6肾切除术或假手术后8周对大鼠进行了研究。这表明尿毒症动物和对照动物之间175种细菌OTU的丰度存在显著差异，最显著的是乳杆菌科和普雷沃氏菌科的减少。因此，尿毒症深刻地改变了肠道微生物组的组成。这一现象的生物影响尚不清楚，有待进一步调查。Kidney International（2013）83，308-315; doi：10.1038/ki.2012.345; 2012年9月19日在线发表
The population of microbes (microbiome) in the intestine. is a symbiotic ecosystem conferring trophic and protective functions. Since the biochemical environment shapes the structure and function of the microbiome, we tested whether uremia and/or dietary and pharmacologic interventions in chronic kidney disease alters the microbiome. To identify different microbial populations, microbial DNA was isolated from the stools of 24 patients with end-stage renal disease (ESRD) and 12 healthy persons, and analyzed by phylogenetic microarray. There were marked differences in the abundance of 190 bacterial operational taxonomic units (OTUs) between the ESRD and control groups. OTUs from Brachybacterium, Catenibacterium, Enterobacteriaceae, Halomonadaceae, Moraxellaceae, Nesterenkonia, Polyangiaceae, Pseudomonadaceae, and Thiothrix families were markedly increased in patients with ESRD. To isolate the effect of uremia from inter-individual variations, comorbid conditions, and dietary and medicinal interventions, rats were studied 8 weeks post 5/6 nephrectomy or sham operation. This showed a significant difference in the abundance of 175 bacterial OTUs between the uremic and control animals, most notably as decreases in the Lactobacillaceae and Prevotellaceae families. Thus, uremia profoundly alters the composition of the gut microbiome. The biological impact of this phenomenon is unknown and awaits further investigation. Kidney International (2013) 83, 308-315; doi:10.1038/ki.2012.345; published online 19 September 2012