Lignin biosynthesis in sugarcane is affected by low temperature
Lignin biosynthesis in sugarcane is affected by low temperature
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DOI：
10.1016/j.envexpbot.2015.08.001
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发表时间：
2015-12-01
影响因子：
5.7
通讯作者：
Mazzafera, Paulo
中科院分区：
文献类型：
--
作者：
dos Santos, Adriana Brombini;Bottcher, Alexandra;Mazzafera, Paulo
甘蔗是生产生物燃料的丰富和有前途的植物生物量来源。生物质转化为乙醇的利用受到木质纤维材料阻力的限制，这主要是由于木质素的存在。本研究对生长在低温(CL‘)和高温(HT-Reference)下的两个甘蔗品种的茎中木质化的某些方面进行了研究。幼嫩和成熟的茎是分开的，茎由皮层(皮)和髓(髓)分开。茎中木质素含量较高的IACSP04-627的植株在高温下的生长(茎鲜重)好于IACSP04-065。低温对植株生长有负面影响，但IACSPO4-065对低温更为敏感。IACSP04-627植株幼皮中木质素含量显著增加，但与单木醇生物合成相关基因的表达谱无直接相关性。Ct处理降低了IACSP04-065植株成熟果皮中的木质素含量，这可能与ShCAD2、ShCOMT1和ShCCoAOMT1基因的低表达水平有关。ShF5H的高表达主要发生在两个甘蔗品种的成熟髓中，这可能与低温诱导的次生细胞壁提前形成有关。综上所述，低温下甘蔗木质素的积累似乎在果皮和果髓组织中受到不同程度的调控，而且是由基因型决定的。(C)2015爱思唯尔B.V.保留所有权利。
Sugarcane is an abundant and promising source of plant biomass for biofuel production. The use of biomass for conversion to ethanol is limited by the recalcitrance of lignocellulosic material mainly due to the presence of lignin. This study characterised some aspects of lignification in the stem of two sugarcane genotypes grown under low (Cl') and warmer (HT - reference) temperatures. Stems were separated in young and mature culms and the culms were separated in cortex (rind) and medulla (pith). Plants of the genotype IACSP04-627, which has more lignin in the stem, grew better (fresh weight of the stem) than IACSP04-065 under HT. Cold negatively affected plant growth but apparently IACSPO4-065 was more sensitive than the other genotype. Lignin content was significantly increased in the young rind of IACSP04-627 plants at CT, what could not be directly correlated with the expression profile of genes of the monolignols biosynthesis. Lignin content in mature rind was reduced in IACSP04-065 plants exposed to CT, what could be correlated with the low expression level of the genes ShCAD2, ShCOMT1 and ShCCoAOMT1. The high expression of ShF5H occurred preferentially in mature pith of both sugarcane genotypes, what is possibly related with the early formation of the secondary cell wall induced by low temperature. In conclusion, lignin deposition in sugarcane under low temperature seems to be differentially regulated in rind and pith tissues and it is genotype-dependent. (C) 2015 Elsevier B.V. All rights reserved.