The addition of endothelial cell growth factor and heparin to human umbilical vein endothelial cell cultures decreases plasminogen activator inhibitor-1 expression.
The addition of endothelial cell growth factor and heparin to human umbilical vein endothelial cell cultures decreases plasminogen activator inhibitor-1 expression.
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在人脐静脉内皮细胞培养物中添加内皮细胞生长因子和肝素可降低纤溶酶原激活剂抑制剂-1 的表达。
DOI：
10.1172/jci113635
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Ginsburg,D
中科院分区：
文献类型：
--
作者：
Konkle,BA;Ginsburg,D
纤溶酶原激活剂抑制剂-1 (PAI-1) 是组织纤溶酶原激活剂 (tPA) 和尿激酶的特异性快速抑制剂。临床研究表明PAI-1可能在纤溶调节中发挥至关重要的作用。许多因素调节内皮细胞培养物中的 PAI-1 活性，现在分离 PAI-1 cDNA 可以在 mRNA 水平上研究 PAI-1 调节。我们检测了内皮细胞生长因子 (ECGF) 和肝素对人脐静脉内皮细胞 (HUVEC) 培养物中 PAI-1 表达的影响。在 HUVEC 培养物中添加 ECGF 和肝素会导致分泌到条件培养基中的 PAI-1 活性降低 3-10 倍。这种效应是在 mRNA 水平上介导的。单独使用较高浓度的 ECGF 时也可以观察到 PAI-1 的降低，但添加肝素后会大大增强。未观察到 tPA 抗原或 mRNA 水平的显着变化。图像
Plasminogen activator inhibitor-1 (PAI-1) is a specific and rapid inhibitor of tissue plasminogen activator (tPA) and urokinase. Clinical studies suggest that PAI-1 may play a crucial role in the regulation of fibrinolysis. A number of factors modulate PAI-1 activity in endothelial cell culture, and the isolation of PAI-1 cDNA now allows study of PAI-1 regulation at the mRNA level. We examined the effect of endothelial cell growth factor (ECGF) and heparin on PAI-1 expression in human umbilical vein endothelial cell (HUVEC) culture. The addition of ECGF and heparin to HUVEC cultures results in a 3-10-fold decrease in the PAI-1 activity secreted into the conditioned media. This effect is mediated at the mRNA level. A decrease in PAI-1 is also seen with higher concentrations of ECGF alone, but is greatly enhanced by the addition of heparin. No significant change in tPA antigen or mRNA levels was observed.Images