INDUCTION OF IMMUNE-RESPONSE AND PROTECTION FROM EQUINE VIRAL ARTERITIS (EVA) BY FORMALIN INACTIVATED-VIRUS VACCINE FOR EVA IN HORSES
INDUCTION OF IMMUNE-RESPONSE AND PROTECTION FROM EQUINE VIRAL ARTERITIS (EVA) BY FORMALIN INACTIVATED-VIRUS VACCINE FOR EVA IN HORSES
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DOI：
10.1111/j.1439-0450.1990.tb01036.x
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发表时间：
1990-03-01
期刊：
影响因子：
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通讯作者：
IMAGAWA, H
中科院分区：
文献类型：
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作者：
FUKUNAGA, Y;WADA, R;IMAGAWA, H
用福尔马林灭活病毒疫苗免疫39匹马，其中包括3匹妊娠母马。马匹单剂免疫后抗体反应较差，灭活前50%有效接种量应包括108.4 pfu的病毒。在间隔4周接种2剂疫苗后，马匹产生了高滴度的SN抗体，滴度高达1：5,120。SN滴度下降较快，但间隔2个月以上补充接种可引起迅速的抗体应答，6个月后SN滴度维持在1：80~1：320。因此，每6个月或1年补充一次疫苗作为加强免疫，将能够维持高滴度的SN抗体。该灭活病毒疫苗预防了马的EVA临床疾病，并通过攻击暴露于强毒病毒来保护怀孕母马免受流产。临床体征和病毒血症证实，SN滴度为1：43的50%保护量。
Thirty-nine horses including 3 pregnant mares were examined by inoculating with formalin inactivated-virus vaccine for EVA. Antibody response of horses after one dose vaccination was somewhat poor and 50% effective inoculum dose of the vaccine should be included 108.4 pfu of virus before inactivation. After 2 doses given at an interval of 4 weeks, the horses developed such high titer of SN antibody as up to 1:5,120. The SN titer declined rather rapidly, but supplemental administration of the vaccine at an interval of more than 2 months elicited a prompt antibody response and SN titers persisted as 1:80 to 1:320 at 6 months after the administration. Therefore, supplemental administration of the vaccine as booster every 6 months or 1 year would be capable of maintaining high titer of SN antibody. The inactivated-virus vaccine prevented horses from clinical disease of EVA and protected pregnant mares from abortion by challenge exposure with virulent virus. Fifty percent protective dose in SN titer of 1:43 was confirmed by clinical signs and viremia.