Effects of Dim Light at Night on Food Intake and Body Mass in Developing Mice.
Effects of Dim Light at Night on Food Intake and Body Mass in Developing Mice.
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DOI：
10.3389/fnins.2017.00294
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发表时间：
2017
影响因子：
4.3
通讯作者：
Nelson RJ
中科院分区：
文献类型：
--
作者：
Cissé YM;Peng J;Nelson RJ
适当定时的光对昼夜节律组织至关重要;夜间暴露于昏暗的光（dLAN）会破坏内源性生物定时的时间组织。成年小鼠暴露于dLAN与体重升高和由摄食时间的自愿变化驱动的代谢变化相关。我们预测幼年小鼠暴露于LAN会影响成年小鼠的代谢功能。在3周龄（实验1）或5周龄（实验2）时，将小鼠维持在标准光-暗（DARK）循环中或暴露于夜间dLAN（5勒克斯）。在前两个实验中，4周后评估食物摄入和自发活动，6周后在实验照明条件下进行葡萄糖耐量试验。在实验3中，以6小时的间隔全天候收集组织，以研究从3或5周龄暴露于dLAN的小鼠中有节奏的肝时钟基因表达。雄性和雌性小鼠暴露于dLAN开始在3周龄表现出类似的增长速度和体重黑暗饲养的后代，尽管增加白天的食物摄入量。暴露于dLAN开始在5周龄增加体重和白天的食物摄入量的男性，但不是女性，小鼠。与体重表型一致，时钟基因表达在肝脏中没有改变。与成年人相比，外周昼夜节律系统发育过程中的dLAN暴露对体重增加具有性别和发育依赖性影响。
Appropriately timed light is critical for circadian organization; exposure to dim light at night (dLAN) disrupts temporal organization of endogenous biological timing. Exposure to dLAN in adult mice is associated with elevated body mass and changes in metabolism putatively driven by voluntary changes in the time of food intake. We predicted that exposure of young mice to LAN could affect adult metabolic function. At 3 weeks (Experiment 1) or 5 weeks (Experiment 2) of age, mice were either maintained in standard light-dark (DARK) cycles or exposed to nightly dLAN (5 lux). In the first two experiments, food intake and locomotor activity were assessed after 4 weeks and a glucose tolerance test was administered after 6 weeks in experimental lighting conditions. In Experiment 3, tissues were collected around the clock at 6 h intervals to investigate rhythmic hepatic clock gene expression in mice exposed to dLAN from 3 or 5 weeks of age. Male and female mice exposed to dLAN beginning at 3 weeks of age displayed similar growth rates and body mass to DARK-reared offspring, despite increasing day-time food intake. Exposure to dLAN beginning at 5 weeks of age increased body mass and daytime food intake in male, but not female, mice. Consistent with the body mass phenotype, clock gene expression was unaltered in the liver. In contrast to adults, dLAN exposure during the development of the peripheral circadian system has sex- and development-dependent effects on body mass gain.