Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome
Orientation of asymmetric stem cell division by the APC tumor suppressor and centrosome
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DOI：
10.1126/science.1087795
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发表时间：
2003-09-12
期刊：
影响因子：
56.9
通讯作者：
Fuller, MT
中科院分区：
文献类型：
--
作者：
Yamashita, YM;Jones, DL;Fuller, MT
干细胞自我更新可以通过周围微环境或小生境的局部信号来指定。然而，小生境和确保干细胞自我更新和分化之间正确平衡的机制之间的关系知之甚少。在这里，我们表明，分裂的果蝇雄性生殖系干细胞使用细胞内机制，涉及中心体功能和皮质定位的腺瘤性结肠息肉病肿瘤抑制蛋白定向有丝分裂纺锤体垂直的利基，确保一个可靠的不对称的结果，其中一个子细胞仍然在利基和自我更新干细胞的身份，而其他的，位移了，启动分化。
Stem cell self-renewal can be specified by local signals from the surrounding microenvironment, or niche. However, the relation between the niche and the mechanisms that ensure the correct balance between stem cell self-renewal and differentiation is poorly understood. Here, we show that dividing Drosophila male germline stem cells use intracellular mechanisms involving centrosome function and cortically localized Adenomatous Polyposis Coli tumor suppressor protein to orient mitotic spindles perpendicular to the niche, ensuring a reliably asymmetric outcome in which one daughter cell remains in the niche and self-renews stem cell identity, whereas the other, displaced away, initiates differentiation.