The R1162 Mob Proteins Can Promote Conjugative Transfer from Cryptic Origins in the Bacterial Chromosome
The R1162 Mob Proteins Can Promote Conjugative Transfer from Cryptic Origins in the Bacterial Chromosome
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DOI：
10.1128/jb.01471-08
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发表时间：
2009-03-01
影响因子：
3.2
通讯作者：
Meyer, Richard
中科院分区：
文献类型：
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作者：
Meyer, Richard
广宿主范围质粒R1162的动员蛋白可以从序列部分简并的19-bp基因座启动质粒的接合转移。这样的基因座很可能偶然出现在细菌染色体中，当R1162存在时，可以作为染色体DNA转移的隐蔽位点。R1162依赖性的染色体DNA转移，从一个这样的潜在网站在黑脓果胶杆菌开始，显示在这里。在大肠杆菌中发现了第二个活性位点，也显示了大量的DNA被转移。这种转移可能反映了染色体中多个隐蔽起源的联合活动。由于细胞质中质粒的存在，染色体DNA的转移描述了以前未被认识到的细菌DNA交换的潜力。
The mobilization proteins of the broad-host-range plasmid R1162 can initiate conjugative transfer of a plasmid from a 19-bp locus that is partially degenerate in sequence. Such loci are likely to appear by chance in the bacterial chromosome and could act as cryptic sites for transfer of chromosomal DNA when R1162 is present. The R1162-dependent transfer of chromosomal DNA, initiated from one such potential site in Pectobacterium atrosepticum, is shown here. A second active site was identified in Escherichia coli, where it is also shown that large amounts of DNA are transferred. This transfer probably reflects the combined activity of the multiple cryptic origins in the chromosome. Transfer of chromosomal DNA due to the presence of a plasmid in the cytoplasm describes a previously unrecognized potential for the exchange of bacterial DNA.