Effect of dietary proteins on the plasma immunoreactive insulin-like growth factor-1/somatomedin C concentration in the rat
Effect of dietary proteins on the plasma immunoreactive insulin-like growth factor-1/somatomedin C concentration in the rat
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膳食蛋白质对大鼠血浆免疫反应性胰岛素样生长因子-1/生长调节素 C 浓度的影响
DOI：
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发表时间：
1990
影响因子：
3.6
通讯作者：
H. Naito
中科院分区：
文献类型：
--
作者：
S. Takahashi;M. Kajikawa;T. Umezawa;S. Takahashi;H. Kato;Y. Miura;T. Nam;T. Noguchi;H. Naito
测定大鼠血浆免疫反应性胰岛素样生长因子-1/生长激素C(IR-IGF-1)的水平，分别饲喂无蛋白饲料、含面筋、添加赖氨酸和苏氨酸的面筋蛋白、添加精氨酸的玉米面筋蛋白、添加色氨酸和赖氨酸的玉米面筋蛋白或酪蛋白。饲喂含酪蛋白120g/kg饲料(4-8U/ml)的大鼠动脉血浆IR-IGF-1浓度高于饲喂无蛋白或低酪蛋白饲料(50g/kg饲料)的大鼠(1.5-2U/ml)。以面筋蛋白或玉米蛋白粉为蛋白质来源的大鼠血浆呈现中间值。然而，按照美国国家研究委员会(National Research Council，1978)的建议，饮食中含有氨基酸混合物，但不含赖氨酸或色氨酸，对血浆IR-IGF-1浓度没有显著影响。饲喂无蛋白饲料的大鼠的总IGF-1浓度(用酸性乙醇提取血浆后进行免疫学测定)也低于饲喂酪蛋白(120g/kg饲料)的大鼠。饲喂酪蛋白饲料(120g酪蛋白/kg饲料)的大鼠IR-IGF-1/总IGF-1的比值高于饲喂无蛋白饲料的大鼠。提示IR-IGF-1或IR-IGF-1：总IGF-1比值对蛋白质合成代谢和营养有重要影响。大鼠血浆经凝胶过滤后，在相对分子质量为40 kDa和150 kDa的组份中检测到IR-IGF-1和总IGF-1。在饲喂酪蛋白饲料的大鼠中，IGF-1在150 kDa的部分得到了较大的恢复。无蛋白饲料喂养的大鼠血浆中加入的125I-IGF-1经凝胶过滤后主要为40 kDa。另一方面，饲喂面筋蛋白或酪蛋白的大鼠血浆中的125I-IGF-1主要以游离IGF-1的形式被回收。结果表明，在无蛋白饲料喂养的大鼠体内，胰岛素样生长因子结合蛋白(S)的相对分子质量约为40 kDa，并未与胰岛素样生长因子1饱和。提示IGF-1及其结合蛋白在大鼠蛋白质代谢调节中具有重要作用。
Plasma immunoreactive insulin-like growth factor-1/somatomedin C (IR-IGF-1) was determined in rats fed for 1 week on a protein-free diet, or diets containing gluten, gluten supplemented with lysine and threonine, maize-gluten meal (with arginine), maize-gluten meal (with arginine) supplemented with tryptophan and lysine, or casein. IR-IGF-1 concentration was higher in the arterial plasma of rats fed on a diet containing casein at 120 g/kg diet (4-8 U/ml) than in rats fed on a protein-free or a low-casein (50 g/kg diet) diet (1.5-2 U/ml). The plasma of rats fed on gluten or maize-gluten meal as the protein source showed intermediate values. However, giving a diet containing an amino acid mixture as recommended by the National Research Council (1978) but deprived of lysine or tryptophan did not affect significantly the plasma IR-IGF-1 concentration. Total IGF-1 concentration (which was measured immunologically after extraction of the plasma with acid-ethanol) was also lower in the rats fed on the protein-free diet than in those fed on the casein (120 g/kg diet) diet. The ratio IR-IGF-1:total IGF-1 was higher in the rats fed on the casein diet (120 g casein/kg diet) than in those fed on the protein-free diet. The results suggest an important influence of IR-IGF-1 or IR-IGF-1:total IGF-1 ratio on protein anabolism and nutrition. IR-IGF-1 and total IGF-1 were found in the fractions of molecular weights 40 kDa and 150 kDa after gel filtration of rat plasma. A larger amount of IGF-1 was recovered in the fraction of 150 kDa in the rats fed on the casein diet. 125I-IGF-1 added to the plasma of rats fed on the protein-free diet was found mainly in the fraction of 40 kDa after gel-filtration. On the other hand, 125I-IGF-1 added to the plasma of rats fed on the gluten or casein diets was mainly recovered in the free IGF-1 fraction. The results suggest that IGF-binding protein(s) of molecular weight about 40 kDa was not saturated with IGF-1 in the rats fed on the protein-free diet. The results indicate the important role of IGF-1 and its binding proteins in the regulation of protein metabolism in rats.
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DOI：
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发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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