Screening for Carbapenemases in Ertapenem-Resistant Enterobacteriaceae Collected at a Tunisian Hospital Between 2014 and 2018
Screening for Carbapenemases in Ertapenem-Resistant Enterobacteriaceae Collected at a Tunisian Hospital Between 2014 and 2018
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DOI：
10.1556/1886.2018.00033
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发表时间：
2019-03-01
影响因子：
2.2
通讯作者：
Ben Moussa, Mohamed
中科院分区：
文献类型：
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作者：
Kollenda, Hans;Frickmann, Hagen;Ben Moussa, Mohamed
背景：在突尼斯患者分离的肠杆菌科中经常检测到碳青霉烯耐药。进行这项研究，以确定频繁的碳青霉烯酶在突尼斯菌株。方法：2014年5月至2018年1月，197厄他培南耐药肠杆菌科分离在突尼斯军事医院的微生物科。对菌株进行表型表征，然后进行靶向碳青霉烯酶基因bla（IMP）、bla（VIM）、bla（NDM）、bla（SPM）、bla（AIM）、bla（DIM）、bla（GIM）、bla（SIM）、bla（KPC）、bla（BIC）和bla（OXA-48）的内部聚合酶链反应（PCR）。评估的197株来自突尼斯的厄他培南耐药肠杆菌科包括170株肺炎克雷伯菌、19株阴沟肠杆菌、6株大肠埃希菌、1株塞氏柠檬酸杆菌和1株阿氏肠杆菌。因此，197株分离株中有55株（27.9%）来自血培养，表明为全身性疾病。碳青霉烯酶基因bla（OXA-48）在数量上占主导地位，有153个检测结果，其次是bla（NDM），有14个检测结果，分布在整个研究期间。与此相反，bla（BIC）和bla（Vim）仅在5例和3例中很少发现，分别，而其他碳青霉烯酶没有observed.Conclusions：碳青霉烯酶基因bla（OXA-48）被确定在绝大多数厄他培南耐药突尼斯肠杆菌科，而所有其他评估的碳青霉烯酶少得多。在数量相关的少数分离株中，应用的基于PCR的筛选方法未鉴别出任何碳青霉烯酶。
Background: Carbapenem-resistance is frequently detected in Enterobacteriaceae isolated from patients in Tunisia. The study was performed to identify frequent carbapenemases in Tunisian isolates.Methods: Between May 2014 and January 2018, 197 ertapenem-resistant Enterobacteriaceae were isolated at the microbiological department of the Military Hospital of Tunis. The strains were phenotypically characterized and then subjected to in-house polymerase chain reaction (PCR) targeting the carbapenemase genes bla(IMP), bla(VIM), bla(NDM), bla(SPM), bla(AIM), bla(DIM), bla(GIM), bla(SIM), bla(KPC), bla(BIC), and bla(OXA-48).Results: The assessed 197 ertapenem-resistant Enterobacteriaceae from Tunis comprised 170 Klebsiella pneumoniae, 19 Enterobacter cloacae, 6 Escherichia coli, 1 Citrobacter sedlakii, and 1 Enterobacter asburiae. Thereby, 55 out of 197 isolates (27.9%) were from blood cultures, suggesting a systemic disease. The carbapenemase gene bla(OXA-48) quantitatively dominated by far with 153 detections, followed by bla(NDM) with 14 detections, which were distributed about the whole study interval. In contrast, bla(BIC) and bla(VIM) were only infrequently identified in 5 and 3 cases, respectively, while the other carbapenamases were not observed.Conclusions: The carbapenemase gene bla(OXA-48) was identified in the vast majority of ertapenem-resistant Tunisian Enterobacteriaceae while all other assessed carbapenemases were much less abundant. In a quantitatively relevant minority of isolates, the applied PCR-based screening approach did not identify any carbapenemases.