The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing
The segment polarity gene porcupine encodes a putative multitransmembrane protein involved in Wingless processing
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DOI：
10.1101/gad.10.24.3116
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发表时间：
1996-12-15
影响因子：
10.5
通讯作者：
Perrimon, N
中科院分区：
文献类型：
--
作者：
Kadowaki, T;Wilder, E;Perrimon, N
Wnt蛋白Wingless（Wg）在果蝇胚胎和成虫盘的图案形成中起信号作用。豪猪（porc）活性的缺乏与类似于wg突变的突变表型有关。在孔突变胚胎中，Wg蛋白仅限于产生它的细胞，这表明Porc在表达或分泌Wg中起作用。PORC编码一种新的跨膜蛋白，其似乎集中在内质网。我们目前的遗传和体外证据表明，孔是专门参与加工的WG。我们鉴定了与Porc相关的人类序列，表明存在参与Wnt加工的蛋白质家族。
The Wnt protein Wingless (Wg) functions as a signal in patterning of both the Drosophila embryo and imaginal discs. Lack of porcupine (porc) activity is associated with mutant phenotypes similar to those of wg mutations. In pore mutant embryos, Wg protein is confined to the cells that produce it, suggesting that Porc plays a role in professing or secretion of Wg. porc encodes a novel transmembrane protein that appears to be concentrated at the endoplasmic reticulum. We present both genetic and in vitro evidence demonstrating that pore is involved specifically in the processing of Wg. We identified a human sequence related to Porc suggesting the existence of a family of proteins involved in processing of Wnts.