In ovo nanoinjection of nonylphenol affects embryonic development of a transgenic see-through medaka (Oryzias latipes), olvas-GFP/STII-YI strain.
In ovo nanoinjection of nonylphenol affects embryonic development of a transgenic see-through medaka (Oryzias latipes), olvas-GFP/STII-YI strain.
复制标题
DOI：
10.1016/j.chemosphere.2009.09.036
复制
发表时间：
2009-12
期刊：
影响因子：
8.8
通讯作者：
Takeshi Hano;Y. Oshima;M. Kinoshita;Minoru Tanaka;Y. Wakamatsu;K. Ozato;Mohamed Nassef;Y. Shimasaki;T. Honjo
中科院分区：
文献类型：
--
作者：
Takeshi Hano;Y. Oshima;M. Kinoshita;Minoru Tanaka;Y. Wakamatsu;K. Ozato;Mohamed Nassef;Y. Shimasaki;T. Honjo
我们将4-壬基酚（NP）纳米注射到具有两个基因型性别标记的转基因水稻（Oryziaslatipes） olass - gfp /STII-YI菌株的胚胎中，并研究了其对发育和性别分化的影响。该转基因由一个绿色荧光蛋白（GFP）基因融合到medaka vasa基因的调控区域组成。生殖细胞特异性GFP表达通过活鱼的透明体壁在生殖腺中可见。分别注射2.0、10、50、125、250ng的NP后，观察每个胚胎的发育情况。NP给药导致大于或等于50ngegg−1的死亡率显著升高，并抑制胚胎发育，包括除10ngegg−1组外的所有治疗组的异常孵化和游泳失败。但对育后10d的生殖细胞增殖和育后100d的生殖细胞性别分化均无不良影响。我们得出结论，单剂量壬基酚暴露在卵中影响了medaka的胚胎发育，但在10dph时影响性腺发育，在100dph时影响成鱼的性别分化。虽然还需要进一步的研究来阐明该菌株胚胎纳米注射的有效性，但目前的研究表明，使用olvas-GFP/STII-YI菌株medaka的纳米注射模型具有用于评估化学物质对早期发育和性别分化的影响的潜力。
We performed in ovo nanoinjection of 4-nonylphenol (NP) into embryos of a transgenic see-through medaka (Oryziaslatipes), olvas-GFP/STII-YI strain, which has two genotypic sex markers, and examined the effects on development and sexual differentiation. The transgene consisted of a green fluorescent protein (GFP) gene fused to the regulatory region of the medaka vasa gene. Germ cell-specific GFP expression was visualized in the gonad through the transparent body wall of the living fish. The development of each embryo was observed after nanoinjection of 2.0, 10, 50, 125, or 250ng of NP. NP administration caused significant higher mortality at⩾50ngegg−1and inhibited embryonic development, including abnormal hatch and swim-up failure in all treatment groups except 10ngegg−1group. However, it did not cause adverse effects on germ cell proliferation by 10d posthatch (dph) or sex differentiation of survivors by 100dph. We concluded that single-dose in ovo exposure to nonylphenol affected embryonic development in the medaka but not gonadal development by 10dph or sexual differentiation in adult fish by 100dph. Although further investigations might be needed to elucidate the usefulness of nanoinjection of embryos of this strain, present study indicated that the nanoinjection model using olvas-GFP/STII-YI strain medaka has potential for use in evaluating the effects of chemicals on early development and sexual differentiation.