Clonal expansion of V gamma 3/V delta 3-expressing gamma delta T cells in an HIV-1/2-negative patient with CD4 T-cell deficiency
Clonal expansion of V gamma 3/V delta 3-expressing gamma delta T cells in an HIV-1/2-negative patient with CD4 T-cell deficiency
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DOI：
10.1046/j.1365-2141.1997.d01-2027.x
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发表时间：
1997-02-01
影响因子：
6.5
通讯作者：
Hoelzer, D
中科院分区：
文献类型：
--
作者：
Kabelitz, D;Hinz, T;Hoelzer, D
对1例HIV-1/2// htlv -1阴性的CD4 T细胞缺乏（0.357-0.6 × 10(9)/l）、γ δ T细胞扩增（占外周血淋巴细胞的26-42%）患者进行免疫学检查，观察3年。用一组可用的V γ /V δ特异性单克隆抗体进行流式细胞术分析表明，病理扩增的γ δ群体在表面表达V γ 2或V γ 3与V δ 3配对，但缺乏CD38或CD71等活化抗原的表达。用V γ γ -C γ和V δ -C δ特异性引物扩增cDNA后获得的RT-PCR产物的克隆和测序证实，该患者外周血中存在克隆扩增的V γ 3/V δ 3群体。以纯化的γ δ T细胞作为效应器和静息或预激活的自体CD4 T细胞作为靶标进行的细胞毒性试验未能揭示V γ 3/V δ 3细胞的自身反应性细胞毒性是该患者CD4 T细胞缺乏的可能机制。
Immunological investigations were carried out in an HIV-1/2//HTLV-1-negative patient with CD4 T-cell deficiency (0.357-0.6 x 10(9)/l) and expansion of gamma delta T cells which accounted for 26-42% of peripheral blood lymphocytes during an observation period of 3 years. Flow cytometry analyses with a panel of available V gamma/V delta-specific monoclonal antibodies indicated that the pathologically expanded gamma delta population expressed V gamma 2 or V gamma 3 paired with V delta 3 on the surface but lacked the expression of activation antigens such as CD38 or CD71. Cloning and sequencing of RT-PCR products obtained after amplification of cDNA with V gamma C gamma and V delta-C delta specific primers confirmed the presence of a clonally expanded V gamma 3/V delta 3 population in the peripheral blood of this patient. Cytotoxicity assays performed with purified gamma delta T cells as effecters and resting or preactivated autologous CD4 T cells as targets failed to reveal evidence for autoreactive cytotoxicity of V gamma 3/V delta 3 cells as a possible mechanism of CD4 T-cell deficiency in this patient.