Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages (Retracted Article)
Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages (Retracted Article)
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DOI：
10.1002/mabi.200700127
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发表时间：
2008-06-11
影响因子：
4.6
通讯作者：
Jabbari, Esmaiel
中科院分区：
文献类型：
--
作者：
Henderson, James A.;He, Xuezhong;Jabbari, Esmaiel
将大鼠骨髓基质（BMS）细胞植入排列的I型胶原支架并在成骨培养基中培养时，它们同时成熟并分化为成骨和血管细胞系。此外，这些细胞产生矿化的基质细胞沉积物。将BMS细胞接种于皮氏培养皿或胶原支架中，在成骨培养基中培养3、6和9 d，随后进行免疫组织化学和细胞化学分析。使用共聚焦显微镜观察谱系特异性蛋白的免疫定位，并通过实时定量聚合酶链反应（RT-qPCR）进行mRNA转录分析。在成骨培养基中，碱性磷酸酶活性和钙含量显著增加。胶原支架内可见大量的Pecam （CD31）、Flk-1 （VEGFR-2）、番茄凝集素（TL/LEL）和α -平滑肌肌动蛋白（α - sma）阳性细胞。在成骨培养中，成骨细胞中也可见新生的毛细血管样血管，促进了BMS细胞向成骨细胞的成熟和分化。在我们的体外研究中，具有多谱系分化潜力的异质细胞群BMS细胞同时分化为成骨和血管谱系，表明底物（三维（3-D）， 1型胶原，排列原纤维）对引导BMS细胞分化途径具有深远的影响。
When rat bone marrow stromal (BMS) cells were seeded on aligned type I Collagen scaffolds and cultured in osteogenic media, they underwent simultaneous maturation and differentiation into osteogenic and vascular cell lineages. In addition, these cells produced mineralized matricellular deposits. BMS cells were seeded in Petri dish or the Collagen scaffold, cultured in osteogenic media for 3, 6, and 9 d and subsequently processed for immunohistochemical and cytochemical analysis. Immunolocalization of lineage-specific proteins were visualized using confocal microscopy and mRNA transcript analysis was performed by real-time quantitative polymerase chain reaction (RT-qPCR). The alkaline phosphatase activity and calcium content significantly increased over the observed period of time in an osteogenic medium. Sheets of abundant Pecam (CD31), Flk-1 (VEGFR-2), tomato lectin (TL/LEL), and alpha-smooth muscle actin (alpha-SMA) positive cells were observed in the Collagen scaffolds. Nascent capillary-like vessels were also seen amidst the osteoblasts in osteogenic culture, augmenting the maturation and differentiation of BMS cells into osteoblasts. In our in vitro study, concurrent differentiation of BMS cells, a heterogeneous cell population with multilineage differentiation potential, to osteogenic and vascular lineages demonstrated that the substrates (three-dimensional (3-D), collagen type 1, aligned fibrils) had a profound effect on guiding the differentiation pathway of BMS cells.