Diurnal changes in lung tumor clearance and their relation to NK cell cytotoxicity in the blood and spleen.
Diurnal changes in lung tumor clearance and their relation to NK cell cytotoxicity in the blood and spleen.
复制标题
肺肿瘤清除率的昼夜变化及其与血液和脾脏中 NK 细胞细胞毒性的关系。
DOI：
10.1002/ijc.1477
复制
发表时间：
2001
影响因子：
6.4
通讯作者：
Ben-Eliyahu,S
中科院分区：
文献类型：
--
作者：
Shakhar,G;Bar-Ziv,I;Ben-Eliyahu,S
据报道，自然杀伤细胞的细胞毒性（NKCC）表现出昼夜节律，在人类血液和大鼠脾脏中的觉醒期间达到峰值。使用F344大鼠，我们研究了这种波动是否（i）反映了NK细胞数量或每个细胞的细胞毒性的变化;（ii）与血液和脾脏一致;（iii）与NK敏感性肿瘤细胞从肺中的清除相对应，以及（iv）影响肺转移的形成。两组大鼠以相反的12：12小时光照方案饲养。在光照或黑暗开始后2小时，两组均处死或静脉接种肿瘤细胞，以研究以下指标：脾脏（n= 29）和血液（n= 79）中的NKCC和NK细胞数量，肿瘤细胞的肺清除率（n= 142）和肺转移（n= 69）。所采用的肿瘤MADB 106是一种仅转移至肺的NK敏感性乳腺腺癌。结果表明，在黑暗期，脾脏NKCC增加（裂解单位[LU]50增加37%），主要是由于脾脏中NK细胞百分比增加28.9%。相比之下，血液NKCC下降了42.5%（LU 20），这种下降与循环NK细胞数量无关，循环NK细胞数量保持恒定。在黑暗中肺肿瘤清除率增加（接种后9小时降低42%），但3周后未观察到转移灶数量的相应变化。我们得出结论，大鼠NKCC的昼夜变化具有器官特异性，涉及细胞分布和活性的变化，并可能影响NK杀肿瘤活性的短期体内指标。© 2001 Wiley利斯公司
Natural killer cell cytotoxicity (NKCC) was reported to manifest a circadian rhythm, peaking during wakefulness in both human blood and rat spleen. Using F344 rats, we investigated whether such fluctuations(i)reflect changes in NK cell numbers or in cytotoxicity per cell;(ii)coincide in the blood and spleen;(iii)correspond with clearance of NK‐sensitive tumor cells from the lungs and(iv)influence formation of lung metastases. Two rat groups were housed in opposite 12:12 hr lighting regimens. Two hours after the onset of light or dark, both groups were either sacrificed or intravenously inoculated with tumor cells to study the following indices: NKCC and NK cell numbers in the spleen (n= 29) and blood (n= 79), lung clearance of tumor cells (n= 142) and lung metastasis (n= 69). The tumor employed, MADB106, is an NK‐sensitive mammary adenocarcinoma that metastasizes only to the lungs. The results indicated that, during the dark phase, splenic NKCC increased (37% higher lytic unit [LU]50) mostly due to a 28.9% higher percentage of NK cells in the spleen. In contrast, blood NKCC decreased by 42.5% (LU20) and this decline was independent of circulating NK cell numbers, which remained constant. Lung tumor clearance increased in the dark (up to 42% lower retention 9 hr after inoculation), but no corresponding changes in the number of metastases were observed 3 weeks later. We conclude that diurnal changes in rats' NKCC are organ‐specific, involve changes in both cell distribution and activity and may affect short‐termin vivoindices of NK tumoricidal activity. © 2001 Wiley‐Liss, Inc.