Spore differentiation by isolated Dictyostelium discoideum cells, triggered by prior cell contact.
Spore differentiation by isolated Dictyostelium discoideum cells, triggered by prior cell contact.
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由分离的盘基网柄菌细胞进行孢子分化，由先前的细胞接触触发。
DOI：
10.1111/j.1432-0436.1978.tb00976.x
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
Maurice Sussman
中科院分区：
文献类型：
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作者：
D. K. Wilcox;Maurice Sussman
细胞D. discoideum突变体Fr-17被允许形成多细胞聚集体并不受干扰地发育12小时（终末形态发生和细胞分化所需的18小时）。然后，将细胞解聚并以低密度重新沉积，以防止进一步持续的细胞接触，并在某些可扩散代谢物的存在下孵育。在这种条件下，它们以正常的时间转化为孢子和柄细胞，在孢子的情况下，比例接近未受干扰的子实体中观察到的比例。相比之下，在早期阶段从聚集体分散的突变细胞或在任何阶段从聚集体分散的野生型细胞在相同条件下保持为变形虫。发现由分离的细胞完成细胞分化需要可扩散、可透析代谢物的阈值浓度。通过加入10 mM NH 4Cl，特别是与氨基酸混合物一起加入，可以满足部分要求。然而，至少一种代谢物必须由通过透析膜或通过增加测试细胞本身的群体密度与测试细胞分离的饲养细胞提供。
Cells of D. discoideum mutant Fr-17 were allowed to form multicellular aggregates and develop undisturbed through 12 h (out of 18-required for terminal morphogenesis and cytodifferentiation). Then the cells were disaggregated and redeposited at densities so low as to preclude further sustained cell contacts and were incubated in the presence of certain diffusible metabolites. In this condition they transformed into spores and stalk cells with normal timing and, in the case of the spores, in proportions approaching those observed in undisturbed fruiting bodies. In contrast, mutant cells dispersed from aggregates at earlier stages or wild type cells dispersed from aggregates at any stage, remained as amoebae under the same conditions. The completion of cytodifferentiation by the isolated cells was found to require threshold concentrations of diffusible, dialysable metabolites. A part of this requirement could be satisfied by addition of 10 mM NH4Cl particularly in conjunction with an amino acid mixture. At least one metabolite, however, had to be supplied by feeder cells separated from the test cells by a dialysis membrane or by increasing the population density of the test cells themselves.