A site‐specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross‐over sites cix for the inversion of the C segment.
A site‐specific, conservative recombination system carried by bacteriophage P1. Mapping the recombinase gene cin and the cross‐over sites cix for the inversion of the C segment.
复制标题
噬菌体 P1 携带的位点特异性保守重组系统。绘制重组酶基因 cin 和 C 段倒位的交叉位点 cix。
DOI：
10.1002/j.1460-2075.1982.tb01336.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
W. Arber
中科院分区：
文献类型：
--
作者：
S. Iida;J. Meyer;K. Kennedy;W. Arber
噬菌体P1基因组携带一个可逆的C片段，由3kb的独特序列组成，两侧是0.6kb的反向重复序列。对于P1衍生物的插入和缺失突变体，位点特异性重组酶基因cin（用于C倒位）已被定位在C片段附近，cix位点（用于C倒位交叉）已位于反向重复序列的外端。C区段的倒位起生物开关的作用，并控制C区段上携带的负责噬菌体感染性的基因的表达。cin基因产物可促进质粒pBR322上的“准cix”位点与P1 DNA上的cix位点之间的重组。在所得的共整合上形成的结也可以用作cix位点。这一观察结果意味着一个潜在的进化过程，使基因受到DNA倒位作用的生物开关的控制。
The bacteriophage P1 genome carries an invertible C segment consisting of 3‐kb unique sequences flanked by 0.6‐kb inverted repeats. With insertion and deletion mutants of P1 derivatives the site‐specific recombinase gene cin for C inversion) has been mapped adjacent to the C segment and the cix sites (for C inversion cross‐over) have been located at the outside ends of the inverted repeats. Inversion of the C segment functions as a biological switch and controls expression of the gene(s) responsible for phage infectivity carried on the C segment. The cin gene product can promote recombination between a ‘quasi‐ cix’ site on plasmid pBR322 and a cix site on P1 DNA. The junctions formed on the resulting co‐integrate can also serve as cix sites. This observation implies a potential evolutionary process to bring genes under the control of a biological switch acting by DNA inversion.