The fine localization of nucleoside triphosphatase activity in the retina of the frog.
The fine localization of nucleoside triphosphatase activity in the retina of the frog.
复制标题
核苷三磷酸酶活性在青蛙的视网膜中的精细定位。
DOI：
10.1083/jcb.17.2.279
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发表时间：
1963-05
期刊：
影响因子：
--
通讯作者：
CRAIG EL
中科院分区：
文献类型：
--
作者：
SCARPELLI DG;CRAIG EL
利用不同的嘌呤和嘧啶核苷，用Wachstein-Meisel方法在亚微观水平上证明了蛙视网膜中核苷三磷酸酶(NTPase)的活性。在电子显微镜下，镁激活的NTPase定位于外节和内节以及网状层。锥体中的NTPase活性部位弥漫地分布在堆叠的片层双层膜之间的70~80A间隙和包被细胞质中。另一方面，在棒中，在堆叠的片层的外围观察到活动的位置，作为离散的电子不透明沉积，其大小为1000到1500安，在片层之间短距离地交错。在波长为510微米的单色光刺激下，暗适应蛙的杆状反应产物出现更多的沉积。在杆状和锥形椭球的线粒体中也观察到了酶的活性。在外丛状层和内丛状层，NTPase活性部位存在于轴突和部分神经元的质膜上或之间。
Nucleoside triphosphatase (NTPase) activity was demonstrated at the submicroscopic level in the frog retina by the Wachstein-Meisel method utilizing various purine and pyrimidine nucleosides. Under the electron microscope magnesium-activated NTPase was localized in the outer and inner segments, and in the plexiform layers. NTPase active sites in the cones were localized diffusely in the 70 to 80 A interspaces between the double membranes of the stacked lamellae and in the investing cytoplasm. In the rods, on the other hand, sites of activity were observed at the periphery of the stacked lamellae as discrete electron opaque deposits measuring 1000 to 1500 A which interdigitated between the lamellae for short distances. Deposits of reaction product appeared more numerous in rods of dark-adapted frogs stimulated with monochromatic light with a wave length of 510 mµ. Enzyme activity was also observed in mitochondria of the rod and cone ellipsoids. In the outer and inner plexiform layers NTPase active sites were present on and between the membranes of axons and the plasma membranes of some of the neurons.