Horizontal Bilayer for Electrical and Optical Recordings
Horizontal Bilayer for Electrical and Optical Recordings
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DOI：
10.3390/ma5122705
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发表时间：
2012-12-01
期刊：
影响因子：
3.4
通讯作者：
Wagner, Richard
中科院分区：
文献类型：
--
作者：
Bartsch, Philipp;Walter, Claudius;Wagner, Richard
含有重组离子通道、转运蛋白和泵的人工双层膜是研究膜蛋白结构与功能关系的电生理学模型。适当构造的微芯片含有水平取向的双层，易于解决方案访问双方提供，此外，有可能调查这些模型双层膜和其中的膜蛋白与高分辨率荧光技术的单分子水平。在这里，我们描述了一个双层微芯片系统，其中可以形成长期稳定的水平独立和水凝胶支持的双层，并证明其前景，特别是单分子荧光光谱和高分辨率荧光显微镜在探测的物理化学性质，如相行为的双层形成脂质，以及在膜蛋白的功能研究。
Artificial bilayer containing reconstituted ion channels, transporters and pumps serve as a well-defined model system for electrophysiological investigations of membrane protein structure-function relationship. Appropriately constructed microchips containing horizontally oriented bilayers with easy solution access to both sides provide, in addition, the possibility to investigate these model bilayer membranes and the membrane proteins therein with high resolution fluorescence techniques up to the single-molecule level. Here, we describe a bilayer microchip system in which long-term stable horizontal free-standing and hydrogel-supported bilayers can be formed and demonstrate its prospects particularly for single-molecule fluorescence spectroscopy and high resolution fluorescence microscopy in probing the physicochemical properties like phase behavior of the bilayer-forming lipids, as well as in functional studies of membrane proteins.