Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice
Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice
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DOI：
10.1038/nbt1173
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发表时间：
2006-01-01
影响因子：
46.9
通讯作者：
Matsuoka, M
中科院分区：
文献类型：
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作者：
Sakamoto, T;Morinaka, Y;Matsuoka, M
具有非常直立的叶片的新品种，增加了光合作用的光捕获和籽粒灌浆的氮储存，可能增加了籽粒产量（1）。在这里，我们表明，直立叶表型水稻油菜素内酯缺乏突变体，osdwarf 4 -1，与提高粮食产量的密植条件下，即使没有额外的肥料。分子和生物化学研究表明，两种不同的细胞色素P450，CYP 90 B2/OsDWARF 4和CYP 724 B1/D11，功能冗余的C-22羟基化，油菜素类固醇生物合成的限速步骤。因此，尽管油菜素类固醇在植物生长和发育中起着重要作用，但OsDWARF 4单独突变仅导致油菜素类固醇生物合成和植物形态的有限缺陷。这些结果表明，调节油菜素类固醇生物合成的遗传调节可以改善作物，而不会产生肥料的负面环境影响。
New cultivars with very erect leaves, which increase light capture for photosynthesis and nitrogen storage for grain filling, may have increased grain yields(1). Here we show that the erect leaf phenotype of a rice brassinosteroid-deficient mutant, osdwarf4-1, is associated with enhanced grain yields under conditions of dense planting, even without extra fertilizer. Molecular and biochemical studies reveal that two different cytochrome P450s, CYP90B2/OsDWARF4 and CYP724B1/D11, function redundantly in C-22 hydroxylation, the rate-limiting step of brassinosteroid biosynthesis. Therefore, despite the central role of brassinosteroids in plant growth and development, mutation of OsDWARF4 alone causes only limited defects in brassinosteroid biosynthesis and plant morphology. These results suggest that regulated genetic modulation of brassinosteroid biosynthesis can improve crops without the negative environmental effects of fertilizers.