Expression of gp130 and leukaemia inhibitory factor receptor subunits in adult rat sensory neurones: regulation by nerve injury
Expression of gp130 and leukaemia inhibitory factor receptor subunits in adult rat sensory neurones: regulation by nerve injury
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DOI：
10.1046/j.1471-4159.2002.01101.x
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发表时间：
2002-10-01
影响因子：
4.7
通讯作者：
Thompson, SWN
中科院分区：
文献类型：
--
作者：
Gardiner, NJ;Cafferty, WBJ;Thompson, SWN
白细胞介素 6 (IL-6) 细胞因子家族的成员被认为是成人神经系统对损伤反应的主要介质。通过分析神经损伤前后雄性成年大鼠背根神经节 (DRG) 中两种信号转导受体亚基糖蛋白 130 (gp130) 和白血病抑制因子受体 (LIFR) 的水平和分布，建立了 IL-6 细胞因子与成年感觉神经元相互作用的基础。所有感觉神经元在细胞质和质膜上都表达 gp130 免疫反应性 (IR)。坐骨神经轴突切除术后 14 天，gp130 的水平及其细胞内分布保持不变。 LIFR-IR 基本上不存在于感觉神经元的细胞质和质膜中，但几乎完全局限于核区室。然而，轴切开后，检测到点状细胞质 LIFR-IR，该点状细胞质 LIFR-IR 持续长达 28 天。轴突切除后 2 天，表达感觉神经肽 CGRP 或细胞表面标记物异凝集素 B4 的小直径感觉神经元子集中，细胞质 LIFR 的表达成比例地更高。轴突切除后，gp130 和 LIFR 在同一细胞内区室中共表达，这表明受伤的感觉神经元对 IL-6 细胞因子家族成员的潜在反应性。
Members of the interleukin-6 (IL-6) family of cytokines have been implicated as major mediators of the response of the adult nervous system to injury. The basis for an interaction of IL-6 cytokines with adult sensory neurones has been established by analysing the levels and distribution of the two signal-transducing receptor subunits, glycoprotein 130 (gp130) and leukaemia inhibitory factor receptor (LIFR), in the dorsal root ganglion (DRG) of male adult rats before and following nerve injury. All sensory neurones express gp130-immunoreactivity (IR) in the cytoplasm and on the plasma membrane. Levels of gp130 and its intracellular distribution remained unchanged up to 14 days following sciatic nerve axotomy. LIFR-IR was largely absent from the cytoplasm and plasma membrane of sensory neurones, but confined almost exclusively to the nuclear compartment. However, following axotomy, punctate cytoplasmic LIFR-IR was detected which persisted up to 28 days following axotomy. The expression of cytoplasmic LIFR 2 days post-axotomy was proportionally greater in a subset of small diameter sensory neurones which expressed either the sensory neuropeptide CGRP or the cell surface marker isolectin B4. The coexpression of gp130 and LIFR in the same intracellular compartment following axotomy conveys potential responsiveness of injured sensory neurones to members of the IL-6 family of cytokines.