Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: a histological and electrophysiological evaluation of regenerated nerves
Peripheral nerve regeneration across an 80-mm gap bridged by a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers: a histological and electrophysiological evaluation of regenerated nerves
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DOI：
10.1016/s0006-8993(00)02207-1
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发表时间：
2000-06-23
期刊：
影响因子：
2.9
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
--
作者：
Matsumoto, K;Ohnishi, K;Shimizu, Y
我们使用新型人工神经导管评估了 80 毫米间隙的周围神经再生。该导管由聚乙醇酸（PGA）胶原蛋白管制成，其中填充有层粘连蛋白涂层的胶原纤维。 12 只比格犬在左腓神经的 80 毫米间隙中植入了神经导管。在另外四只用作阴性对照的狗中，神经被切除并保持不连接。组织学观察显示，许多无髓鞘和有髓神经纤维（其直径均比正常神经纤维更小，髓鞘更薄）在植入后 12 个月重新生长穿过间隙并超出间隙。再生轴突直径的分布与正常轴突直径的分布不同。植入后 3 个月，记录大多数动物的复合肌肉动作电位、运动诱发电位和体感诱发电位。峰值幅度和潜伏期逐渐恢复，这表明神经与目标器官的连接功能建立。除了普通的电生理恢复之外，在植入后 12 个月刺激间隙远端的腓神经后，源自 A α、A δ 和 C 纤维的具有不同潜伏期的电位在第 6 腰椎处变得可区分。植入后10-12个月，无负重行走模式几乎恢复正常。对照组中未获得电生理学和组织学恢复。我们的神经导管可以引导周围神经伸长，并在比之前报道的人工神经导管更宽的神经间隙中实现良好的功能恢复。 (C) 2000 Elsevier Science B.V. 保留所有权利。
We evaluated peripheral nerve regeneration across an 80-mm gap using a novel artificial nerve conduit. The conduit was made of a polyglycolic acid (PGA)-collagen tube filled with laminin-coated collagen fibers. Twelve beagle dogs underwent implantation of the nerve conduit across an 80-mm gap in the left peroneal nerve. In four other dogs used as negative controls, the nerve was resected and left unconnected. Histological observation showed that numerous unmyelinated and myelinated nerve fibers, all smaller in diameter and with a thinner myelin sheath than normal nerve fibers, regrew through and beyond the gap 12 months after implantation. The distribution of the regenerated axonal diameters was different from that of the normal axonal diameters. Compound muscle action potentials, motor evoked potentials, and somatosensory evoked potentials were recorded in most animals 3 months after implantation. Peak amplitudes and latencies recovered gradually, which indicating the functional establishment of the nerve connection with the target organs. In addition to the ordinary electrophysiological recoveries, potentials with distinct latencies originating from A alpha, A delta and C fibers became distinguishable at the 6th lumbar vertebra following stimulation of the peroneal nerve distal to the gap 12 months after implantation. The pattern of walking without load was restored to almost normal 10-12 months after implantation. Neither electrophysiological nor histological restoration was obtained in the controls. Our nerve conduit can guide peripheral nerve elongation and lead to favorable functional recovery across a wider nerve gap than previously reported artificial nerve conduits. (C) 2000 Elsevier Science B.V. All rights reserved.