Macrophage colony-stimulating factor and granulocyte colony-stimulating factor separated from fibrosarcoma tissue in mice.
Macrophage colony-stimulating factor and granulocyte colony-stimulating factor separated from fibrosarcoma tissue in mice.
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从小鼠纤维肉瘤组织中分离出巨噬细胞集落刺激因子和粒细胞集落刺激因子。
DOI：
10.20772/cancersci1959.75.4_355
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Kunitake Hirashima
中科院分区：
文献类型：
--
作者：
N. Sakai;Mikio Shikita;K. Tsuneoka;Masami Bessho;Kunitake Hirashima;Kunitake Hirashima
在患有纤维肉瘤的小鼠血液中发现粒细胞和单核细胞大量增加。用等渗盐水提取肿瘤组织，产生集落刺激因子（CSF）。用0.1%十二烷基硫酸钠进一步提取盐不溶性物质，得到另一池脑脊液。肿瘤细胞的体外培养导致CSF活性在培养基中积累。这些来源的CSF在小鼠骨髓细胞培养中产生粒细胞集落和巨噬细胞集落。随后，通过等电聚焦和重复凝胶过滤层析将产生粒细胞集落的活性与产生巨噬细胞集落的活性分离。抗l细胞CSF抗血清能中和巨噬细胞CSF活性，但不能中和粒细胞CSF活性。这些结果表明粒细胞诱导肿瘤产生两种类型的脑脊液。
Large increases of granulocytes and monocytes were found in the blood of mice bearing fibrosarcoma. Extraction of the tumor tissue with isotonic saline yielded a colony-stimulating factor (CSF). Further extraction of the saline-insoluble materials with 0.1% sodium dodecyl sulfate afforded another pool of CSF. Incubation of the tumor cells in vitro resulted in the accumulation of CSF activity in the culture medium. The CSF from these sources produced both granulocyte colonies and macrophage colonies in murine bone marrow cell culture. Subsequently, the activity producing granulocyte colonies was separated from that producing macrophage colonies by isoelectrofocusing and repeated gel-filtration chromatography. It was also shown that anti-L cell CSF antiserum neutralized the macrophage CSF activity but not the granulocyte CSF activity. These results show that the granulocytosis-inducing tumor produces two types of CSF.
集落刺激因子 (CSF-1) 的纯化、测定、表征和靶细胞结合的方法。
影响因子：
2.2
作者：
Stanley,ER;Guilbert,LJ
通讯作者：
Guilbert,LJ