Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis
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DOI：
10.1038/s41598-020-61507-z
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发表时间：
2020-03-25
影响因子：
4.6
通讯作者：
Hermenegildo, Carlos
中科院分区：
文献类型：
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作者：
Mompeon, Ana;Ortega-Paz, Luis;Hermenegildo, Carlos
尽管miRNAs在心血管疾病（CVD）的诊断和预后方面具有很好的价值，但最近的荟萃分析并不支持其潜力。研究中的方法学差异可能会干扰miRNA谱并影响其结果。本研究通过对血浆和血清中与CVD相关的主要miRNA表达进行配对比较，确定血液起始材料是否是miRNA谱变异的来源。血浆和血清中的循环miRNA产量相似，尽管与对照志愿者相比，在非ST段抬高型心肌梗死（NSTEMI）患者中观察到显著增加。当通过miR-484的表达进行标准化时，血清和血浆之间的miRNA表达模式不同。尽管NSTEMI改变了血清和血浆中miR-1和miR-208的表达，但血浆显示出比血清更高的变异性（Levene检验p
Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profile and affect their results. This study determines if the blood starting material is a source of variance in miRNA profile by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a significant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, different patterns of miRNA expression between serum and plasma. Although NSTEMI modified the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p