Dominant prevalence of Porphyromonas gingivalis fimA types I and IV in healthy Japanese children.
Dominant prevalence of Porphyromonas gingivalis fimA types I and IV in healthy Japanese children.
复制标题
健康日本儿童中牙龈卟啉单胞菌 fimA I 型和 IV 型的主要患病率。
DOI：
10.1016/j.jds.2017.03.006
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
and Nemoto TK.
中科院分区：
文献类型：
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作者：
Shimoyama Y;Ohara-Nemoto Y;Kimura M;Tanaka M; Kimura S;and Nemoto TK.
背景/目的牙龈卟啉单胞菌是慢性牙周炎的主要致病菌，但其感染环境尚不清楚。为了检测儿童牙菌斑中携带不同fimA型细菌的患病率，我们建立了适用于有限数量标本的PCR方法。通过这种方法，所有六种fimA型，包括I型和Ib型，直接确定，和流行ofimA型和他们的频率在日本childhen.Materials和methodsGenomic DNA纯化牙菌斑标本牙周健康的儿童（2-12岁，4.8 ± 0.2岁）和19个母亲的牙龈卟啉单胞菌阳性的儿童科目。采用巢式PCR法对第一次PCR分析中无法分型的菌株进行基因分型。牙龈炎发生率为15.2%（2-10岁，5.1 ± 0.6岁），以I型和IV型最多见（各37.0%），其次为Ib型和III型（各11.1%），最后为II型（7.4%）。20 P中7例（35.0%）。牙龈炎阳性的受试者有I型和其他A型的混合定植。在大多数情况下，在儿童的细菌患病率和fimA型是不同的，从他们的母亲，表明其母婴传播是不显着的conclusionThese结果表明，非疾病相关fimA型I和IVP的定植。牙龈炎从儿童早期开始，但没有显示任何牙周炎症。
Background/purposePorphyromonas gingivalisis a major causative agent of chronic periodontitis, whilst circumstances for acquisition of the bacterium remain to be elucidated. To examine prevalence of the bacterium harboring distinctfimAtypes in dental plaque of children, we established PCR procedures that are applicable for specimens with limited amounts. By this method, all sixfimAtypes including type I and Ib were directly identified, and prevalence offimAtypes and their frequency of guardian-child transmission in Japanese children were assessed.Materials and methodsGenomic DNA was purified from dental plaque specimens of 132 periodontally healthy children (2–12 years old, 4.8 ± 0.2 years) and 19 mothers of resultantP. gingivalis-positive child subjects. PCR-basedfimAgenotyping was performed, and untypeable strains in the first PCR analysis were determined by a nested PCR.ResultsP. gingivaliswas found in 15.2% of the subjects (2–10 years old, 5.1 ± 0.6 years), and the most prevalent types were I and IV (37.0% each), followed by Ib and III (11.1% each), and then II (7.4%). Seven (35.0%) of the 20P. gingivalis-positive subjects had combined colonization of type I with otherfimAtypes. In most cases, bacterial prevalence andfimAtypes in the children were distinct from those of their mothers, indicating that its maternal transmission was not significant.ConclusionThese results suggest that colonization of non-disease-associatedfimAtypes I and IVP. gingivalisto the oral cavity initiates from early childhood without showing any periodontal inflammation.