Effect of Hemoperfusion Using Polymyxin B-Immobilized Fiber on IL-6, HMGB-1, and IFN Gamma in a Neonatal Sepsis Model
Effect of Hemoperfusion Using Polymyxin B-Immobilized Fiber on IL-6, HMGB-1, and IFN Gamma in a Neonatal Sepsis Model
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DOI：
10.1203/01.pdr.0000169995.25333.f7
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发表时间：
2005-08
影响因子：
3.6
通讯作者：
M. Hussein;Takenori Kato;T. Sugiura;G. A. Daoud;Satoshi Suzuki;S. Fukuda;H. Sobajima;I. Kato;H. Togari
中科院分区：
文献类型：
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作者：
M. Hussein;Takenori Kato;T. Sugiura;G. A. Daoud;Satoshi Suzuki;S. Fukuda;H. Sobajima;I. Kato;H. Togari
为了评估多粘菌素B直接血液灌流（PMX-DHP）对新生儿脓毒症盲肠结扎穿孔（CLP）模型的影响，在24头麻醉和机械通气的3龄仔猪中，16头在CLP后3 h至6 h进行CLP和动静脉体外循环，PMX-DHP治疗组（8头）有pmx柱，8头为假手术。在CLP前、3、9 h测定血浆脂多糖（LPS），在CLP前、1、3、6、6 h测定血清平均体血压（mSBP）、平均肺动脉压、血清IL-6、肿瘤坏死因子α、干扰素γ和高流动性组-1盒蛋白的变化。和9 h。有限合伙人是虚假的和PMX-DHP组低于控制在9 h。mSBP骗局和PMX-DHP组高于控制在6 h。il - 6是虚假的和PMX-DHP组低于控制在6 h。HMGB-1 PMX-DHP组低于在控制在6 h。干扰素-γ才发现在对照组9 h。PMX-DHP组生存时间长于在控制。因此，PMX-DHP可改善新生儿脓毒性休克模型。
To evaluate effects of polymyxin B direct hemoperfusion (PMX-DHP) on a neonatal sepsis cecal ligation and perforation (CLP) model, in 24 anesthetized and mechanically ventilated 3-d-old piglets, 16 were assigned to CLP and an arteriovenous extracorporeal circuit from 3 h until 6 h post-CLP, with a PMX-column in PMX-DHP–treated group (8 piglets) and 8 as sham. Plasma lipopolysaccharide (LPS) was measured at before CLP and at 3 and 9 h. Changes in mean systemic blood pressure (mSBP), mean pulmonary blood pressure, serum IL-6, tumor necrosis factor alpha, interferon gamma, and highly mobile group-1 box protein were measured before CLP and at 1, 3, 6, and 9 h. LPS was lower in the sham and PMX-DHP groups than in the control at 9 h. The mSBP was higher in the sham and PMX-DHP groups than in the control at both 6 h. IL-6 was lower in the sham and PMX-DHP groups than in the control at 6 h. HMGB-1 was lower in the PMX-DHP group than in the control at 6 h. IFN-γ was only detected in the control group at 9 h. Survival times in the PMX-DHP group were longer than in the control. Thus, PMX-DHP improved septic shock in a neonatal septic model.