Overexpression of miRNA-21 promotes radiation-resistance of non-small cell lung cancer.
Overexpression of miRNA-21 promotes radiation-resistance of non-small cell lung cancer.
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miRNA-21过表达促进非小细胞肺癌的放射抗性
DOI：
10.1186/1748-717x-8-146
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发表时间：
2013-06-19
期刊：
影响因子：
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通讯作者：
Luo JC
中科院分区：
文献类型：
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作者：
Wang XC;Wang W;Zhang ZB;Zhao J;Tan XG;Luo JC
背景 此前有报道称 MiRNA-21 在多种癌症中表达上调。本研究旨在探讨miRNA-21在非小细胞肺癌中的潜在作用。材料与方法采用RT-qPCR检测60例非小细胞肺癌（NSCLC）样本及癌旁正常组织中miRNA-21的表达，并利用统计软件分析miRNA-21表达与NSCLC临床病理特征的相关性。检测miRNA-21表达对辐射诱导的A549细胞生长和凋亡的影响。结果非小细胞肺癌中miRNA-21表达增加。 miRNA-21的表达与淋巴结转移、临床分期和不良预后呈正相关。多变量Cox回归分析显示miRNA-21是患者的独立预后因素。 miRNA-21 的下调抑制了 A549 细胞的增殖和细胞周期进程，并使细胞对辐射敏感。 miRNA-21表达的减少促进了辐射诱导的A549细胞的凋亡。结论miRNA-21可能被认为是未来开发非小细胞肺癌特异性治疗干预的潜在新靶点。
BackgroundMiRNA-21 was previously reported to be up-regulated in many kinds of cancer. In the present study, we want to investigate the potential role of miRNA-21 in non-small cell lung cancer.Materials and methodsExpression of miRNA-21 was detected in 60 non-small cell lung cancer (NSCLC) samples and adjacent histologically normal tissue using RT-qPCR, Correlation between miRNA-21 expression and clinicopathological features of NSCLC was analyzed using statistical software. The effect of miRNA-21 expression on the growth and apoptosis of A549 cells induced by irradiation was examined.ResultsmiRNA-21 expression increased in non-small cell lung cancer. Expression of miRNA-21 was positively associated with lymph node metastasis, clinical stage and poor prognosis. Multivariate Cox regression analysis showed that miRNA-21 was an independent prognostic factor for patients. Down-regulation of miRNA-21 inhibited proliferation and cell cycle progress of A549 cells and sensitized cells to radiation. Decreased miRNA-21 expression promoted the apoptosis of A549 cells induced by irradiation.ConclusionsmiRNA-21 may be considered as a potential novel target for future development of specific therapeutic interventions in NSCLC.