Dihydrorhodamine 123 is superior to 2,7-dichlorodihydrofluorescein diacetate and dihydrorhodamine 6G in detecting intracellular hydrogen peroxide in tumor cells
Dihydrorhodamine 123 is superior to 2,7-dichlorodihydrofluorescein diacetate and dihydrorhodamine 6G in detecting intracellular hydrogen peroxide in tumor cells
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DOI：
10.1016/j.cellbi.2007.08.028
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发表时间：
2008-02-01
影响因子：
3.9
通讯作者：
Gong, Xingguo
中科院分区：
文献类型：
--
作者：
Qin, Yong;Lu, Min;Gong, Xingguo
研究了二氢达拉明123 （DHR 123）、2,7-二氯二氢荧光素二乙酸酯（DCFH-DA）和二氢达拉明6G （DHR 6G）作为检测SPC-A-1肺腺癌细胞过氧化氢水平的探针。成像技术和荧光激活细胞扫描用于探针反应的研究。暴露25 min后可见明显的绿色荧光。MitoTracker Orange CM-H2TMRos（线粒体探针）和上述探针同时染色后，只有DHR 123和DHR 6G组在线粒体区域显示出清晰的绿色荧光。DHR 6G组的荧光强度较弱。将100 μ M H2O2加入到负载探针的SPC-A-1细胞中，细胞内荧光迅速显著增加。结果表明，DHR 123在SPC-A-1细胞过氧化氢的瞬时检测中具有优势。(C) 2007年国际细胞生物学联合会。Elsevier Ltd.出版。版权所有。
Dihydrorhodamine 123 (DHR 123), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), and dihydrorhodamine 6G (DHR 6G) were evaluated as probes for detecting cellular hydrogen peroxide levels in SPC-A-1 lung adenocarcinoma cells. Imaging techniques and fluorescence-activated cell scan were used in the study of the probe responses. Obvious green fluorescence was established after a 25-min exposure. After staining with MitoTracker Orange CM-H2TMRos (a probe for mitochondria) and the abovementioned probes simultaneously, only the DHR 123 and DHR 6G groups exhibited legible green fluorescence in the mitochondrial regions. Furthermore, the DHR 6G group exhibited weaker fluorescence intensity. When 100 mu M H2O2 was added to SPC-A-1 cells loaded with these probes, the intracellular fluorescence increased rapidly and significantly. Our results suggest that DHR 123 is superior for the instantaneous detection of cellular hydrogen peroxide in SPC-A-1 cells. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.