Changes of microbial characteristics of retained sludge during low-temperature operation of an EGSB reactor for low-strength wastewater treatment.
Changes of microbial characteristics of retained sludge during low-temperature operation of an EGSB reactor for low-strength wastewater treatment.
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EGSB反应器处理低浓度废水低温运行过程中滞留污泥微生物特性的变化
DOI：
10.2166/wst.2008.077
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
A. Ohashi
中科院分区：
文献类型：
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作者：
K. Syutsubo;W. Yoochatchaval;H. Yoshida;K. Nishiyama;M. Okawara;H. Sumino;N. Araki;H. Harada;A. Ohashi
在实验室规模的EGSB反应器上运行了400天，考察了降温对剩余污泥微生物特性的影响。EGSB反应器在15℃的温度下启动，接种20℃的颗粒污泥。模拟废水进水COD控制在0.6~0.8gCOD/L，运行400天，工艺温度由15℃逐步降至5℃。反应器温度从15℃降至10℃时，COD的去除效率下降。然而，EGSB反应器的连续运行使废水在10℃下得到了有效的处理(COD去除70%，甲烷回收率50%)。我们证实，在15~20℃的温度下，剩余污泥的醋酸盐补给和氢气补给的产甲烷活性都明显提高。结果表明，甲烷螺菌属产氢产甲烷菌的增殖是由于反应器的低温运行所致。另一方面，在整个实验过程中，甲烷菌属以产乙酸菌的形式大量存在。
In this study, a lab scale EGSB reactor was operated for 400 days to investigate the influence of temperature-decrease on the microbial characteristic of retained sludge. The EGSB reactor was started-up at 15 degrees C seeding with 20 degrees C-grown granular sludge. The influent COD of synthetic wastewater was set at 0.6-0.8 gCOD/L. The process-temperature was stepwise reduced from 15 degrees C to 5 degrees C during 400 days operation. Decrease of temperature of the reactor from 15 degrees C to 10 degrees C caused the decline of COD removal efficiency. However, continuous operation of the EGSB reactor led the efficient treatment of wastewater (70% of COD removal, 50% of methane recovery) at 10 degrees C. We confirmed that the both acetate-fed and hydrogen-fed methanogenic activities of retained sludge clearly increased under 15 to 20 degrees C. Changes of microbial profiles of methanogenic bacteria were analyzed by 16S rDNA-targeted DGGE analysis and cloning. It shows that genus Methanospirillum as hydrogen-utilizing methanogen proliferated due to low temperature operation of the reactor. On the other hand, genus Methanosaeta presented in abundance as acetoclastic-methanogen throughout the experiment.
嗜热颗粒污泥中厌氧微生物群落响应饲料成分变化的种群动态
DOI：
--
发表时间：
2001
期刊：
Water Science and Technology 43・1
影响因子：
--
作者：
Shutsubo K.;Sinthurat N.;Ohashi A.;Harada H.
通讯作者：
Harada H.