Photo-Crosslinkable Polymeric Coatings Providing Chemically Versatile Reactive Surfaces on Various Substrates
Photo-Crosslinkable Polymeric Coatings Providing Chemically Versatile Reactive Surfaces on Various Substrates
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DOI：
10.1021/acs.chemmater.3c00225
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发表时间：
2023-04-28
影响因子：
8.6
通讯作者：
Kim, Myungwoong
中科院分区：
文献类型：
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作者：
Choi, Seungyeon;Yutzy, Lane D.;Kim, Myungwoong
我们展示了一种化学路线，以获得简单，清洁，可扩展，可重复图案化，化学可调，和多功能的系统，以制造反应性有机薄膜。它们的表面在各种表面类型上表现出有效的生物相容性、受控的反应性和可加工性，即，具有各种功能的可光交联的聚合物薄膜，其进一步适用于限定用于界面连接生物物体的表面活性。用三种单体合成共聚物：含聚（乙二醇）的单体，在暴露于UV光时释放伯胺的单体，以及具有与胺高度反应性的环状二硫代碳酸酯或缩水甘油基的单体。所得共聚物用极性溶剂如水或醇处理用于涂布，并且在UV光照射下容易交联以形成高度稳定的分子网络，这有利于在各种有机和无机基底上的所需区域中限定选择性反应性表面。所设计的系统是化学上通用的固定生物相关分子的表面上的（i）后交联改性，通过反应的牛血清白蛋白上的剩余的表面反应性基团与伯胺的蛋白质和（ii）预交联制剂与反应性明胶，具有交联的功能，在复合薄膜。我们进一步表明，在玻璃或聚苯乙烯基底上得到的涂层提供了优异的生物相容性和C2C12鼠心肌细胞的生长。
We demonstrate a chemical route to attain simple, clean, scalable, photopatternable, chemically adjustable, and versatile systems to fabricate reactive organic thin films. Their surfaces exhibit effective biocompatibility, controlled reactivity, and processability on various surface types, i.e., photo-crosslinkable polymeric thin films with various functionalities, further applicable for the definition of surface activity for interfacing biological objects. The copolymers were synthesized with three monomers: a poly(ethylene glycol)-containing monomer, a monomer releasing a primary amine upon exposure to UV light, and a monomer bearing cyclic dithiocarbonate or glycidyl groups that are highly reactive with the amine. The resulting copolymers were processed with polar solvents such as water or alcohol for coating and were readily crosslinked under UV light illumination to form a highly stable molecular network, beneficial for defining selective reactive surfaces in a desired region on a variety of organic and inorganic substrates. The designed system was chemically versatile in immobilizing biologically relevant molecules on the surfaces by (i) postcrosslinking modification through the reaction of bovine serum albumin on the remaining surface reactive group with a primary amine in the protein and (ii) pre-crosslinking formulation with reactive gelatin that has the functionality to crosslink in the composite thin film. We further showed that the resulting coating on a glass or polystyrene substrate provided excellent biocompatibility and growth of C2C12 murine myocytes.