Cross-talk between airway and gut microbiome links to IgE responses to house dust mites in childhood airway allergies
Cross-talk between airway and gut microbiome links to IgE responses to house dust mites in childhood airway allergies
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呼吸道和肠道微生物组之间的串扰与儿童呼吸道过敏中对屋尘螨的IgE反应有关
DOI：
10.1038/s41598-020-70528-7
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发表时间：
2020-08-10
影响因子：
4.6
通讯作者：
Su, Shih-Chi
中科院分区：
文献类型：
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作者：
Chiu, Chih-Yung;Chan, Yi-Ling;Su, Shih-Chi
儿童过敏中与过敏原暴露相关的气道和肠道微生物群之间的联系尚未得到很好的解决。我们的目的是确定患有气道过敏的幼儿中与螨特异性 IgE 反应相关的气道和肠道微生物群变化。这项研究招募了 60 名儿童，其中包括 38 名螨虫过敏儿童（20 名鼻炎儿童和 18 名哮喘儿童）和 22 名非螨虫过敏的健康对照。使用细菌 16S rRNA 测序对咽拭子和粪便样本进行微生物组组成分析。对与儿童过敏性鼻炎和哮喘 IgE 反应相关的气道和粪便微生物谱进行综合分析。气道中的 Chao1 指数和 Shannon 指数显着低于粪便中的指数。此外，还注意到气道微生物多样性与屋尘螨（HDM）致敏和过敏性气道疾病呈负相关。粪便 IgE 水平与血清中屋尘螨和粉尘螨特异性 IgE 水平呈正相关。气道细毛菌属与哮喘相关的细菌与粪便中的Dorea和瘤胃球菌属密切相关，它们与粪便中的IgE水平和过敏性鼻炎的风险呈负相关。此外，四个气道属，弯曲杆菌属、硒单胞菌属、坦纳菌属和阿托波菌属，与血清螨特异性和粪便 IgE 水平呈负相关。其中，气道月孢单胞菌和阿托波菌属。与粪便中的 Blautia 和 Dorea spp 呈正相关。分别与哮喘和过敏性鼻炎有关。总之，HDM 引起的气道微生物失调及其与肠道微生物群落的相互作用与儿童早期过敏性气道疾病有关。
A connection between airway and gut microbiota related to allergen exposure in childhood allergies was not well addressed. We aimed to identify the microbiota alterations in the airway and gut related to mite-specific IgE responses in young children with airway allergies. This study enrolled 60 children, including 38 mite-sensitized children (20 rhinitis and 18 asthma), and 22 non-mite-sensitized healthy controls. Microbiome composition analysis of the throat swab and stool samples was performed using bacterial 16S rRNA sequencing. An integrative analysis of the airway and stool microbial profiling associated with IgE reactions in childhood allergic rhinitis and asthma was examined. The Chao1 and Shannon indices in the airway were significantly lower than those in the stool. Additionally, an inverse association of the airway microbial diversity with house dust mite (HDM) sensitization and allergic airway diseases was noted. Fecal IgE levels were positively correlated with the serum Dermatophagoides pteronyssinus- and Dermatophagoides farinae-specific IgE levels. Airway Leptotrichia spp. related to asthma were strongly correlated with fecal Dorea and Ruminococcus spp., which were inversely associated with fecal IgE levels and risk of allergic rhinitis. Moreover, four airway genera, Campylobacter, Selenomonas, Tannerella, and Atopobium, were negatively correlated with both serum mite-specific and fecal IgE levels. Among them, the airway Selenomonas and Atopobium spp. were positively correlated with stool Blautia and Dorea spp. related to asthma and allergic rhinitis, respectively. In conclusion, airway microbial dysbiosis in response to HDM and its cross-talk with the gut microbial community is related to allergic airway diseases in early childhood.