Structure and expression of the cell division genes ftsQ, ftsA and ftsZ.
Structure and expression of the cell division genes ftsQ, ftsA and ftsZ.
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细胞分裂基因 ftsQ、ftsA 和 ftsZ 的结构和表达。
DOI：
10.1016/0022-2836(85)90290-6
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发表时间：
1985
影响因子：
5.6
通讯作者：
Lutkenhaus,J
中科院分区：
文献类型：
--
作者：
Yi,QM;Rockenbach,S;WardJr,JE;Lutkenhaus,J
细胞分裂必需基因ftsQ、ftsA和ftsZmap位于大肠杆菌遗传图谱上两分钟处的一簇细胞被膜基因中，似乎构成了一个非典型操纵子。这些紧密聚集的基因都以相同的方向转录，然而这些基因中的每一个都被独立地克隆到多拷贝质粒上并被发现表达。该区域的Tn5插入突变证实了这些基因是独立表达的，表明这些基因中的每一个都有自己的启动子。然而，最大表达的distalftsZ基因需要的proximalftsQ基因的启动子。ftsZ启动子位于ftsA结构基因内，ftsA启动子位于ftsQ结构基因内。从该区域的DNA序列分析中没有明显的转录终止子。序列分析还表明，ftsQ的终止密码子与ftsA基因的起始密码子重叠，ftsA和ftsZ基因相隔60个碱基对。FtsQ基因产物的计算Mr为31，432，FtsA基因产物的计算Mr为45，327。这些基因的结构和表达进行了讨论。
The essential cell division genesftsQ,ftsAandftsZmap in a cluster of cell envelope genes located at two minutes on theEscherichia coligenetic map and appear to constitute an atypical operon. These closely clustered genes are all transcribed in the same direction and yet each of these genes was independently cloned on a multicopy plasmid and found to be expressed. Tn5 insertion mutagenesis of this region confirmed that these genes were independently expressed, indicating that each of these genes has its own promoter. However, maximum expression of the distalftsZgene required the promoter for the proximalftsQgene. The proximalftsZpromoter was located within theftsAstructural gene and the proximalftsApromoter was located within theftsQstructural gene. No transcription terminators were evident from the DNA sequence analysis of this region. The sequence analysis also revealed that the termination codon forftsQoverlapped the initiation codon of theftsAgene and that theftsAandftsZgenes were separated by 60 base-pairs. TheftsQgene product has a calculatedMrof 31,432 and theftsAgene product has a calculatedMrof 45,327. The structure and expression of these genes are discussed.