CHLAMYDIA-TRACHOMATIS DEVELOPMENTALLY REGULATED PROTEIN IS HOMOLOGOUS TO EUKARYOTIC HISTONE H1
CHLAMYDIA-TRACHOMATIS DEVELOPMENTALLY REGULATED PROTEIN IS HOMOLOGOUS TO EUKARYOTIC HISTONE H1
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DOI：
10.1073/pnas.88.9.3937
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发表时间：
1991-05-01
影响因子：
11.1
通讯作者：
YING, Y
中科院分区：
文献类型：
--
作者：
HACKSTADT, T;BAEHR, W;YING, Y
衣原体是原核专性细胞内寄生虫，其经历双相生命周期，涉及称为原体的感染性细胞外形式和称为网状体的细胞内复制形式。 我们已经从沙眼衣原体中纯化了一种非常基本的基本体特异性蛋白，其表观分子量为18 kDa，确定了其N-末端氨基酸序列，并克隆了编码基因。 克隆基因的序列分析表明，其表达产物具有一些显着的特性，包括高赖氨酸含量（29%），相应的高pI，并显着同源性的真核细胞组蛋白的H-1类。 此外，这种衣原体组蛋白类似物的单克隆抗体，被称为HC 1，显示免疫印迹和抗核特异性提示的交叉反应H-1组蛋白。 该基因只在衣原体生命周期的后期阶段，伴随着重组的衣原体网状体成基本机构，表明HC 1蛋白在细胞内分化的衣原体染色质的凝聚中发挥作用。
Chlamydiae are prokaryotic obligate intracellular parasites that undergo a biphasic life cycle involving an infectious, extracellular form known as elementary bodies and an intracellular, replicating form termed reticulate bodies. We have purified from Chlamydia trachomatis a very basic elementary body-specific protein with an apparent molecular mass of 18 kDa, determined its N-terminal amino acid sequence, and cloned the encoding gene. Sequence analysis of the cloned gene revealed some remarkable properties for its expressed product, including a high lysine content (29%), a correspondingly high pI, and significant homology to the H-1 class of eukaryotic histones. Furthermore, a monoclonal antibody to this chlamydial histone analog, termed Hc1, displayed immunoblot and antinuclear specificity suggestive of cross-reactivity to H-1 histones. The gene was expressed only during the late stages of the chlamydial life cycle concomitant with the reorganization of chlamydial reticulate bodies into elementary bodies, suggesting that the Hc1 protein plays a role in the condensation of chlamydial chromatin during intracellular differentiation.