Subgingival microbiota of chronic periodontitis subjects from different geographic locations
Subgingival microbiota of chronic periodontitis subjects from different geographic locations
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DOI：
10.1111/j.1600-051x.2004.00597.x
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发表时间：
2004-11-01
影响因子：
6.7
通讯作者：
Socransky, SS
中科院分区：
文献类型：
--
作者：
Haffajee, AD;Bogren, A;Socransky, SS
背景：大多数临床研究假设不同地理位置的牙龈下微生物群是相似的。本研究的目的是研究来自四个国家的慢性牙周炎患者牙龈下微生物群的组成。方法：招募慢性牙周炎患者（瑞典101例，美国115例，巴西58例，智利26例）。在基线时测量受试者每颗牙齿6个部位的牙菌斑、牙龈炎、探探出血（BOP）、化脓、牙袋深度（PD）和附着水平（AL）。采用棋盘DNA-DNA杂交技术分别对基线时每颗牙齿内侧的龈下菌斑样本进行40种细菌含量的分析（总样本=6036）。由每个物种组成的DNA探针计数为每个位点确定，并在每个受试者的每个位点上取平均值。使用ANCOVA对年龄、平均口袋深度、性别和吸烟状况进行调整，确定各国之间每种物种比例差异的显著性。对多重比较的p值进行了调整。结果：平均而言，在四个国家的受试者样本中检测到所有物种。在调整了多次比较后，13种物种在各国之间的调整后平均比例也存在显著差异。牙龈卟啉单胞菌（Porphyromonas龈卟啉单胞菌）在不同国家的比例不同，在巴西、智利、瑞典和美国的受试者中，其微生物群的调整平均值分别为7.5%、11.9%、1.6%和6.6%
Background: Most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. The purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries.Method: Subjects with chronic periodontitis (N, Sweden=101; USA=115; Brazil=58; Chile=26) were recruited. Subjects were measured at baseline for plaque, gingivitis, bleeding on probing (BOP), suppuration, pocket depth (PD) and attachment level (AL) at six sites per tooth. Subgingival plaque samples taken from the mesial aspect of each tooth at baseline were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization (total samples=6036). % DNA probe counts comprised by each species was determined for each site and averaged across sites in each subject. Significance of differences in proportions of each species among countries was determined using ANCOVA adjusting for age, mean pocket depth, gender and smoking status. p-Values were adjusted for multiple comparisons.Results: On average, all species were detected in samples from subjects in the four countries. Thirteen species differed significantly in adjusted mean proportions among countries even after adjusting for multiple comparisons. Porphyromonas gingivalis, one species that differed in proportions among countries, comprised adjusted means of 7.5, 11.9, 1.6 and 6.6% of the microbiota in subjects from Brazil, Chile, Sweden and USA (p