Expression and function of Toll-like receptors in human basophils
Expression and function of Toll-like receptors in human basophils
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DOI：
10.1159/000092707
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Yamaguchi, Masao
中科院分区：
文献类型：
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作者：
Komiya, Akiko;Nagase, Hiroyuki;Yamaguchi, Masao
我们研究了人类嗜碱性粒细胞中一组 Toll 样受体 (TLR) 的表达和功能。嗜碱性粒细胞制剂组成型表达 TLR2、TLR4、TLR9 和 TLR10 mRNA（TLR4 > TLR2 >> TLR9、TLR10）。尽管与中性粒细胞和单核细胞相比，嗜碱性粒细胞中的 TLR mRNA 表达通常不太显着，但嗜碱性粒细胞中 TLR2 和 TLR4 mRNA 的表达水平明显高于嗜酸性粒细胞。测试了各种 TLR 配体（Pam(3)Cys-Ser-Lys(4)、Poly I:C、脂多糖、R-848、CpG DNA），但均不影响粘附分子 CD11b 的表达水平或新鲜纯化的嗜碱性粒细胞的活力。另一方面，当在用 TLR 配体刺激之前用干扰素-γ 预处理嗜碱性粒细胞时，只有 TLR4 配体脂多糖上调 CD11b 表达。然而，干扰素-γ处理的嗜碱性粒细胞表面的TLR2和TLR4水平没有表现出明显的变化。这些结果表明嗜碱性粒细胞上的TLR4可能通过调节嗜碱性粒细胞功能参与感染引起的过敏性炎症恶化的发病机制。版权所有 (c) 2006 S. Karger AG，巴塞尔。
We investigated the expression and function of a panel of Toll-like receptors (TLRs) in human basophils. Basophil preparations constitutively expressed TLR2, TLR4, TLR9 and TLR10 mRNAs (TLR4 > TLR2 >> TLR9, TLR10). Although TLR mRNA expression in basophils was generally less prominent compared with those in neutrophils and monocytes, basophils expressed significantly higher levels of TLR2 and TLR4 mRNA than eosinophils. Various TLR ligands (Pam(3)Cys-Ser-Lys(4), Poly I:C, lipopolysaccharide, R-848, CpG DNA) were tested, but none affected the expression level of adhesion molecule CD11b or the viability of freshly purified basophils. On the other hand, when basophils were pretreated with interferon-gamma before stimulation with TLR ligands, only the TLR4 ligand, lipopolysaccharide, upregulated CD11b expression. However, the surface levels of TLR2 and TLR4 on the interferon-gamma-treated basophils showed no obvious changes. These results suggest that TLR4 on basophils may be involved in the pathogenesis of infection-induced exacerbation of allergic inflammation by modulating basophil functions. Copyright (c) 2006 S. Karger AG, Basel.