Macrophage-derived chemokine (MDC)/CCL22 produced by monocyte derived dendritic cells reflects the disease activity in patients with atopic dermatitis
Macrophage-derived chemokine (MDC)/CCL22 produced by monocyte derived dendritic cells reflects the disease activity in patients with atopic dermatitis
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DOI：
10.1016/j.jdermsci.2006.08.004
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发表时间：
2006-11-01
影响因子：
4.6
通讯作者：
Tamaki, Kunihiko
中科院分区：
文献类型：
--
作者：
Hashimoto, Shinichi;Nakamura, Koichiro;Tamaki, Kunihiko
背景：特应性皮炎是一种反复发作的炎症性皮肤病，以血清中高水平的IgE和Th2型细胞因子如IL-4、IL-5或IL-13为特征。趋化因子吸引炎症组织中的白细胞。我们已发现AD患者血浆中高水平分泌胸腺和活化调节趋化因子(TARC)/CCL17和巨噬细胞衍生趋化因子(MDC)/CCL22。树突状细胞(DC)是一种抗原提呈细胞，分为单核细胞来源的DC(MoDC)和浆细胞样树突状细胞(PDCs)。目的：研究AD患者、寻常型银屑病(PSV)患者和正常对照(HC)MoDC产生CCL17和CCL22的情况。结果：AD患者MODC产生的CCL22水平明显高于PSV患者和HC患者。MODC产生的CCL22水平与SCORAD指数显著相关。在AD患者、PSV患者和HC患者之间，MODC产生的CCL17水平没有显著差异。免疫抑制剂地塞米松(Dex)、他克莫司和环孢素(Cys)可抑制AD患者MODC产生CCL22。结论：MODC产生的CCL22水平反映了AD的疾病活动性，可能在AD的发病机制中起重要作用。(C)2006年日本皮肤病研究学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Backgrounds: Atopic dermatitis (AD) is a recurrent inflammatory skin disease characterized by high serum levels of IgE and Th2-type cytokines such as IL-4, IL-5 or IL-13. Chemokines attract leukocytes in inflamed tissues. We have previously found that thymus and activation regulated chemokine (TARC)/CCL17 and macrophage-derived chemokine (MDC)/CCL22 are highly secreted in the plasma levels of AD patients. Dendritic cells (DCs) are antigen-presenting cells that are divided into two subgroups including monocyte derived DCs (MoDCs) and plasmacytoid DCs (pDCs).Objectives: The aim of the study was to elucidate CCL17 and CCL22 production by MoDCs in AD patients, psoriasis vulgaris (PsV) patients and healthy controls (HC).Methods: MoDCs were obtained from AD patients, PsV patients or HC and were cultured. In addition, the chemokine levels were measured in the supernatants.Results: We found that the CCL22 levels produced by MoDCs in AD patients to be significantly higher than those of PsV patients and HC. There was a significant correlation between the CCL22 levels produced by MoDCs and the SCORAD index. No significant difference in the CCL17 levels produced by MoDCs was detected among AD patients, PsV patients or HC. Immunosuppressive drugs such as dexamethasone (Dex), tacrolimus and cyclosporine (Cys) inhibited the CCL22 production by MoDCs in the AD patients.Conclusion: These data suggest that the CCL22 level produced by MoDCs thus reflects the disease activity of AD and it may also play an important role regarding the production of CCL22 in the pathogenesis of AD. (c) 2006 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.