Analysis of the butyrate-producing pathway in Porphyromonas gingivalis.

Analysis of the butyrate-producing pathway in Porphyromonas gingivalis.
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牙龈卟啉单胞菌丁酸产生途径的分析。

DOI:
10.1007/978-1-0716-0939-2_16
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发表时间:
2021
期刊:
In: Nagano K, Hasegawa Y (eds). Periodontal Pathogens: Methods in Molecular Biology.
影响因子:
--
通讯作者:
Yoshida Y.
Yoshida Y.
中科院分区:
--
文献类型:
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作者:
山坂美紗;浅野智志;早田敦子;中澤敬信;橋本 均;Waschek James;吾郷由希夫;磯野俊仁,土門久哲,前川知樹,田村 光,日吉 巧,柳原克紀,國友栄治,寺尾 豊;栗栖諒子,三枝 禎,青野悠里,林 良憲,人見涼露,前田 茂,嶋田昌彦,岩田幸一,篠田雅路;Yoshida Y.

文献摘要

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丁酸盐是口腔中发现的最有害的代谢终产物之一。因此,重要的是要确定负责产生这种代谢物的酶的特性,以阐明牙周病细菌的致病性。本文介绍了丁酰辅酶A:醋酸酯辅酶A转移酶活性的分光光度分析方法,以及丁酸和其他短链脂肪酸如乙酸酯、丙酸酯、异丁酸酯和异戊酸酯的气相色谱-质谱仪测定方法。
Butyrate is one of the most harmful metabolic end products found in the oral cavity. Thus, it would be important to characterize the enzymes responsible for production of this metabolite to elucidate the pathogenicity of periodontogenic bacteria. Here, a spectrophotometric assay for butyryl-CoA:acetate CoA transferase activity and gas chromatography–mass spectrometry measurement of butyrate and other short chain fatty acids such as acetate, propionate, isobutyrate, and isovalerate are described.