Anti-influenza A Virus Activity of Dendrobine and Its Mechanism of Action
Anti-influenza A Virus Activity of Dendrobine and Its Mechanism of Action
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DOI：
10.1021/acs.jafc.7b00276
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发表时间：
2017-05-10
影响因子：
6.1
通讯作者：
Yang, Jie
中科院分区：
文献类型：
--
作者：
Li, Richan;Liu, Teng;Yang, Jie
石斛碱是金钗石斛的主要成分，因其在保健方面的广泛应用而日益受到人们的关注。在这里，我们探讨了石斛碱对甲型流感病毒的潜在影响，并阐明了潜在的机制。我们的研究结果表明，石斛碱具有抗甲型流感病毒，包括A/FM-1/1/47（H1N1），A/波多黎各/8/34 H274 Y（H1N1）和A/爱知/2/68（H3 N2）的抗病毒活性，IC 50值分别为3.39 +/- 0.32，2.16 +/- 0.91，5.32 +/- 1.68 μ g/mL。机制研究表明，石斛碱抑制病毒复制周期的早期步骤。值得注意的是，石斛碱可以结合到病毒核蛋白（NP）的高度保守区域，随后抑制病毒NP的核输出及其寡聚化。总之，石斛碱显示出潜在的开发作为一个有前途的药物治疗流感病毒感染。更重要的是，这一结果为中药“石斛”的充分应用提供了宝贵的信息。
Dendrobine, a major component of Dendrobium nobile, increasingly draws attention for its wide applications in health care. Here we explore potential effects of dendrobine against influenza A virus and elucidate the underlying mechanism. Our results indicated that dendrobine possessed antiviral activity against influenza A viruses, including A/FM-1/1/47 (H1N1), A/Puerto Rico/8/34 H274Y (H1N1), and A/Aichi/2/68 (H3N2) with IC50 values of 3.39 +/- 0.32, 2.16 +/- 0.91, 5.32 +/- 1.68 mu g/mL, respectively. Mechanism studies revealed that dendrobine inhibited early steps in the viral replication cycle. Notably, dendrobine could bind to the highly conserved region of viral nucleoprotein (NP), subsequently restraining nuclear export of viral NP and its oligomerization. In conclusion, dendrobine shows potential to be developed as a promising agent to treat influenza virus infection. More importantly, the results provide invaluable information for the full application of the Traditional Chinese Medicine named "Shi Hu".