Drosophila poised enhancers are generated during tissue patterning with the help of repression
Drosophila poised enhancers are generated during tissue patterning with the help of repression
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DOI：
10.1101/gr.209486.116
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发表时间：
2017-01-01
期刊：
影响因子：
7
通讯作者：
Zeitlinger, Julia
中科院分区：
文献类型：
--
作者：
Koenecke, Nina;Johnston, Jeff;Zeitlinger, Julia
组蛋白修饰经常被用作增强子状态的标记，但如何在胚胎发育的背景下解释增强子状态尚不清楚。据报道，稳定的增强子签名涉及H3K4mel和低水平的H3K27ac，以标记准备未来激活的非活性增强子。然而，未来的激活并不总是被观察到，而且这种增强子签名广泛出现的替代原因还没有被调查。通过分析果蝇胚胎背腹(DV)轴形成过程中的增强子，我们发现稳定的增强子信号是在组织中的图案形成过程中特异性产生的，其中不诱导增强子，包括在已知被转录抑制子抑制的增强子上。这些结果表明，稳定的增强子状态可能是组织构图过程中空间调节的标志，而不是作为未来激活之前的中间步骤。我们讨论了稳定的增强子状态更一般地是转录抑制物抑制的结果的可能性。
Histone modifications are frequently used as markers for enhancer states, but how to interpret enhancer states in the context of embryonic development is not clear. The poised enhancer signature, involving H3K4mel and low levels of H3K27ac, has been reported to mark inactive enhancers that are poised for future activation. However, future activation is not always observed, and alternative reasons for the widespread occurrence of this enhancer signature have not been investigated. By analyzing enhancers during dorsal-ventral (DV) axis formation in the Drosophila embryo, we find that the poised enhancer signature is specifically generated during patterning in the tissue where the enhancers are not induced, including at enhancers that are known to be repressed by a transcriptional repressor. These results suggest that, rather than serving exclusively as an intermediate step before future activation, the poised enhancer state may be a mark for spatial regulation during tissue patterning. We discuss the possibility that the poised enhancer state is more generally the result of repression by transcriptional repressors.