Baseline Meningococcal Carriage in Burkina Faso before the Introduction of a Meningococcal Serogroup A Conjugate Vaccine
Baseline Meningococcal Carriage in Burkina Faso before the Introduction of a Meningococcal Serogroup A Conjugate Vaccine
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DOI：
10.1128/cvi.00479-10
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发表时间：
2011-03-01
影响因子：
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通讯作者：
Caugant, Dominique A.
中科院分区：
文献类型：
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作者：
Kristiansen, Paul A.;Diomande, Fabien;Caugant, Dominique A.
A 群脑膜炎球菌结合疫苗 MenAfriVac 有可能通过减少流行菌株的携带率来赋予群体免疫力。为了更好地了解这一现象，我们启动了一项脑膜炎球菌携带研究，以确定布基纳法索 1 至 29 岁人群（选择首次引入疫苗的群体）在接种疫苗之前的基线携带率和血清群分布。 2009 年，在布基纳法索的一个城市和两个农村地区进行了一项多横断面运输研究。每 3 个月，在 4 周内从超过 5,000 名随机选择的个体中采集口咽样本。布基纳法索国家实验室对 20,326 个样本中的脑膜炎球菌进行了分离和鉴定。在挪威进行了确认和进一步的菌株表征，包括基因分型、多位点序列分型和 porA-fetA 测序。脑膜炎球菌总体携带率为3.98%；男性患病率最高的是15至19岁，女性患病率最高的是10至14岁。 Y 血清群占主导地位 (2.28%)，其次是 X 血清群 (0.44%)、A (0.39%) 和 W135 (0.34%)。农村地区和旱季的携带率最高，但不同地区的血清群分布也有所不同。总共鉴定了 29 种序列类型 (ST) 和 51 种 porA-fetA 组合。优势克隆是血清群 Y、ST-4375、P1.5-1,2-2/F5-8，属于 ST-23 复合体（47%）。所有血清组 A 分离株均为带有 P1.20,9/F3-1 的 ST-5 复合体的 ST-2859。这项研究为评估 MenAfriVac 引入对 A 群血清携带的影响奠定了坚实的基础。
The serogroup A meningococcal conjugate vaccine MenAfriVac has the potential to confer herd immunity by reducing carriage prevalence of epidemic strains. To better understand this phenomenon, we initiated a meningococcal carriage study to determine the baseline carriage rate and serogroup distribution before vaccine introduction in the 1- to 29-year old population in Burkina Faso, the group chosen for the first introduction of the vaccine. A multiple cross-sectional carriage study was conducted in one urban and two rural districts in Burkina Faso in 2009. Every 3 months, oropharyngeal samples were collected from >5,000 randomly selected individuals within a 4-week period. Isolation and identification of the meningococci from 20,326 samples were performed by national laboratories in Burkina Faso. Confirmation and further strain characterization, including genogrouping, multilocus sequence typing, and porA-fetA sequencing, were performed in Norway. The overall carriage prevalence for meningococci was 3.98%; the highest prevalence was among the 15- to 19-year-olds for males and among the 10- to 14-year-olds for females. Serogroup Y dominated (2.28%), followed by serogroups X (0.44%), A (0.39%), and W135 (0.34%). Carriage prevalence was the highest in the rural districts and in the dry season, but serogroup distribution also varied by district. A total of 29 sequence types (STs) and 51 porA-fetA combinations were identified. The dominant clone was serogroup Y, ST-4375, P1.5-1,2-2/F5-8, belonging to the ST-23 complex (47%). All serogroup A isolates were ST-2859 of the ST-5 complex with P1.20,9/F3-1. This study forms a solid basis for evaluating the impact of MenAfriVac introduction on serogroup A carriage.