Investigation of functional selenium nanoparticles as potent antimicrobial agents against superbugs
Investigation of functional selenium nanoparticles as potent antimicrobial agents against superbugs
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功能性硒纳米粒子作为对抗超级细菌的有效抗菌剂的研究
DOI：
10.1016/j.actbio.2015.10.041
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发表时间：
2016-01-15
影响因子：
9.7
通讯作者：
Liu, Jie
中科院分区：
文献类型：
--
作者：
Huang, Xiaoquan;Chen, Xu;Liu, Jie
开发高效的抗菌剂对于广泛的应用具有重要意义。然而，多种耐药细菌的出现对公共卫生构成了威胁。由于化学不稳定、生物相容性低、长期抗菌效果差，许多已开发的药物在实际应用中受到限制。在接下来的研究中，我们将槲皮素（Qu）和乙酰胆碱（Ach）偶联到硒纳米粒子表面，合成了一种协同纳米复合材料（Qu-Ach@SeNPs）。槲皮素具有广泛的生物活性，其抗菌活性与乙酰胆碱作为神经递质在细菌细胞上与受体结合有关。箭头表示NPs，箭头表示受损细胞壁。该研究表明，与单独的Qu@SeNPs和Ach@SeNPs相比，Qu-Ach@SeNPs如何表现出协同增强的抗多药超级细菌（mdr）的抗菌性能。Qu-Ach@SeNPs在低剂量下对耐多药，如耐甲氧西林金黄色葡萄球菌（MRSA）有效。机理研究表明Qu-Ach@SeNPs附着在细菌细胞壁上，对细胞膜造成不可逆的损伤，从而达到显著的协同抑菌作用，抑制MRSA。槲皮素和乙酰胆碱的协同作用增强了SeNPs的抗菌活性。通过这种方式，Qu-Ach@SeNPs包含了一类新的无机纳米抗菌剂，可用于生物医学设备的有用应用。意义说明Qu-Ach@SeNPs在体外正常人细胞上的细胞毒性较低。Qu-Ach@SeNPs在低剂量下对耐多药，如耐甲氧西林金黄色葡萄球菌（MRSA）有效。重要的是，Qu-Ach@SeNPs没有显示出耐药性。结果表明Qu-Ach@SeNPs具有良好的抗菌活性。这些药物可以作为对抗超级细菌的良好抗菌剂。我们的数据表明，这些抗菌剂可能在抗多药耐药引起的传染病以及其他传染病的医学领域有广泛的应用。(C) 2015材料学报Elsevier Ltd.出版。版权所有。
Developing highly effective antibacterial agents is important for a wide range of applications. However, the emergence of multiple antibiotic-resistant bacteria poses a public health threat. Many developed agents have limited practical application due to chemical instability, low biocompatibility, and poor long-term antibacterial efficiency. In the following study, we synthesize a synergistic nanocomposite by conjugating quercetin (Qu) and acetylcholine (Ach) to the surface of Se nanoparticles (Qu-Ach@SeNPs). Quercetin has been reported to exhibit a wide range of biological activities related to their antibacterial activity and acetylcholine as a neurotransmitter, which can combine with the receptor on the bacterial cell. Arrows indicate NPs and arrowheads indicate compromised cell walls. The study demonstrated how Qu-Ach@SeNPs exhibit a synergistically enhanced antibacterial performance against the multidrug-resistant superbugs (MDRs) compared to Qu@SeNPs and Ach@SeNPs alone. Qu-Ach@SeNPs are effective against MDRs, such as Methicillin-resistant Staphylococcus aureus (MRSA), at a low dose. The mechanistic studies showed that Qu-Ach@SeNPs attach to the bacterial cell wall, causing irreversible damage to the membrane, and thereby achieving a remarkable synergistic antibacterial effect to inhibit MRSA. The findings suggested that the synergistic properties of quercetin and acetylcholine enhance the antibacterial activity of SeNPs. In this way, Qu-Ach@SeNPs comprise a new class of inorganic nano-antibacterial agents that can be used as useful applications in biomedical devices.Statement of significanceThe Qu-Ach@SeNPs have low cytotoxicity when tested on normal human cells in vitro. Qu-Ach@SeNPs are effective against MDRs, such as Methicillin-resistant S. aureus (MRSA), at a low dose. Importantly, Qu-Ach@SeNPs showed no emergence of resistance. These results suggest that Qu-Ach@SeNPs have excellent antibacterial activities. These agents can serve as good antibacterial agents against superbugs. Our data suggest that these antibacterial agents may have widespread application in the field of medicine for combating infectious diseases caused by MDRs, as well as other infectious diseases. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.