Real-time 3D single-molecule localization using experimental point spread functions.
Real-time 3D single-molecule localization using experimental point spread functions.
复制标题
实时3D单分子使用实验点扩散函数的定位。
DOI:
10.1038/nmeth.4661
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发表时间:
2018-05
期刊:
影响因子:
48
通讯作者:
Ries J
中科院分区:
文献类型:
--
作者:
Li Y;Mund M;Hoess P;Deschamps J;Matti U;Nijmeijer B;Sabinina VJ;Ellenberg J;Schoen I;Ries J
We present a real-time fitter for 3D single-molecule localization microscopy using experimental point spread functions (PSFs) that achieves optimal 3D resolution on any microscope and is compatible with any PSF engineering approach. This allowed us to image cellular structures with a 3D resolution unprecedented for astigmatic PSFs. The fitter compensates for most optical aberrations and makes accurate 3D superresolution microscopy broadly accessible, even on standard microscopes without dedicated 3D optics.
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